DEMYELINATION IN SPINAL CORD TRAUMA

脊髓创伤中的脱髓鞘

• 批准号: 3401943
• 负责人: ANDREW R BLIGHT
• 金额: $ 10.39万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3401943
• 关键词: action potentials; aminopyridines; axon; disease /disorder model; electrical potential; electron microscopy; histochemistry /cytochemistry; membrane potentials; microelectrodes; model design /development; myelination; neural conduction; neuropharmacology; paraplegia; reticuloendothelial system; spinal cord injury; tetraethylammonium compound; tetrodotoxin; tissue /cell culture

The structure and electrophysiological characteristics of central spinal axons will be determined during successive stages of the inflammatory response that follows spinal cord contusion injury, in cats. The time-course and mechanism of dysfunction in surviving axons will be investigated, using intracellular microelectrode recording and current-injection in an in vitro preparation of spinal cord. The quantitative extent of axonal survival and myelination will be examined with light- and electron-microscopy, for comparison with physiological recordings in the same tissue. The myelination and electrophysiology of selected individual axons will be correlated by electrophoretic injection of horseradish peroxidase at the time of recording, and subsequent processing for microscopy. The time-course and extent of phagocytic infiltration of the tissue, as a possible contributive factor in axonal pathology, will also be examined. The experiments relate to the problem of defining the extent to which functional loss in traumatic paraplegia and paraparesis is be due to factors other than direct destruction of axons, particularly their demyelination and incomplete remyelination. This will address the utility of attempting to develop therapy for these currently untreatable conditions through improvement of remyelination or treatment of demyelination pathophysiology. The study will provide information on the role of the myelin sheath in action potential conduction, and the physiological effects of demyelination and remyelination in the central nervous systems. This basic knowledge will also be of significance outside the particular context of spinal trauma.

中央脊柱的结构和电生理特征 轴突将在炎症的连续阶段确定 猫中脊髓挫伤损伤后的反应。 这 幸存轴突中功能障碍的时间和机制将是 使用细胞内微电极记录和 在体外制备脊髓中的电流注射。 这 将检查轴突生存和髓鞘的定量范围 与光和电子显微镜检查，可与生理学进行比较 在同一组织中的记录。 的髓鞘化和电生理学 选定的单个轴突将通过电泳注射与 记录时的辣根过氧化物酶，随后 处理显微镜。 吞噬细胞的时间和程度 组织的渗透，作为轴突的可能因素 病理学也将进行检查。 实验与 确定创伤性截瘫和 模拟是由于轴突直接破坏以外的其他因素， 特别是他们的脱髓鞘和不完整的恢复性。 这会 解决试图为这些目前开发治疗的实用性 通过改善re髓的治疗或治疗的条件不可治疗 脱髓鞘病理生理学。 该研究将提供有关 髓鞘在动作潜在传导中的作用， 中央脱髓鞘和再生的生理影响 神经系统。 这种基本知识也将具有重要意义 脊柱创伤的特殊情况。