STUDIES ON NEURONAL SPECIFICITY AND PLASTICITY

神经元特异性和可塑性研究

基本信息

批准号：
3401515
负责人：
STEVEN M FREDMAN
金额：
$ 7.36万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-12-01 至 1987-11-30
项目状态：
已结题

项目摘要

The long-term goal of my research is to obtain an understanding of how neurons recognize each other and form specific synaptic connections. The proposed research will examine the specificity and plasticity of chemical monosynaptic connections between identified neurons in the cerebral and pleural ganglia of Aplysia. The neurons make specific monosynaptic connections within each ganglion (intraganglionic connections). and with neurons in the other ganglion (interganglionic connections). Each synaptic connection is a physiological distinct type: fast EPSP, slow EPSP, IPSP. The neurons regenerate following axotomy produced by crushing the cerebro-pleural connectives. This disrupts the interganglionic synaptic connections but not the intraganglionic connections. The neurons in both ganglia will be examined physiologically using intracellular recordings and morphologically by means of intracellular dye injection during and following axonal regeneration. I will determine if regenerating neurons re-establish synaptic connections only with their specific followers or if synapses with inappropriate neurons are formed. The physiological properties of the regenerated synaptic connections (correct and incorrect) will be determined. If correct synaptic connections are found, I will determine if they are stable or subsequently lost as the specific connections are established. The results will help elucidate the mechanisms by which central neurons regenerate and form specific synaptic contacts. I will also examine the intraganglionic connections of the same neurons in each ganglion for plastic changes in synaptic function during the regeneration process. If plastic changes are observed., I will determine the physiological and morphological bases for those changes. I will also determine if the functioning of the synapses returns to normal when the interganglionic synaptic connections are reestablished. This will provide insights into how neurons regulate their synaptic connections.

我研究的长期目标是了解如何神经元相互识别并形成特定的突触连接。这拟议的研究将检验化学的特异性和可塑性大脑中已识别的神经元之间的单突触连接海兔的胸膜神经节。神经元产生特定的单突触每个神经节内的连接（神经节内连接）。并与另一个神经节中的神经元（神经节间连接）。每个突触连接是一种生理上不同的类型：快速 EPSP、慢速 EPSP、IPSP。神经元在轴切开后再生脑-胸膜连接词。这会破坏神经节间突触连接，但不是神经节内连接。两者的神经元将使用细胞内记录对神经节进行生理检查在形态学上通过细胞内染料注射的方式进行轴突再生后。我将确定神经元是否再生仅与其特定追随者重新建立突触连接，或者如果形成具有不适当神经元的突触。生理学再生突触连接的属性（正确和错误）将被确定。如果找到正确的突触连接，我会确定它们是否稳定或随后丢失连接已建立。结果将有助于阐明中枢神经元再生并形成特定突触的机制联系人。我还将检查相同的神经节内连接每个神经节中的神经元在突触功能的可塑性变化再生过程。如果观察到塑料变化，我会确定这些变化的生理和形态学基础。我还将确定突触的功能是否恢复正常当神经节间突触连接重新建立时。这将提供有关神经元如何调节其突触连接的见解。