STUDIES ON NEURONAL SPECIFICITY AND PLASTICITY

神经元特异性和可塑性研究

• 批准号: 3401515
• 负责人: STEVEN M FREDMAN
• 金额: $ 7.36万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3401515
• 关键词: axon; electrophysiology; ganglions; nervous system regeneration; neural information processing; neural plasticity; neuroanatomy; neurophysiology; saltwater environment; synapses

The long-term goal of my research is to obtain an understanding of how neurons recognize each other and form specific synaptic connections. The proposed research will examine the specificity and plasticity of chemical monosynaptic connections between identified neurons in the cerebral and pleural ganglia of Aplysia. The neurons make specific monosynaptic connections within each ganglion (intraganglionic connections). and with neurons in the other ganglion (interganglionic connections). Each synaptic connection is a physiological distinct type: fast EPSP, slow EPSP, IPSP. The neurons regenerate following axotomy produced by crushing the cerebro-pleural connectives. This disrupts the interganglionic synaptic connections but not the intraganglionic connections. The neurons in both ganglia will be examined physiologically using intracellular recordings and morphologically by means of intracellular dye injection during and following axonal regeneration. I will determine if regenerating neurons re-establish synaptic connections only with their specific followers or if synapses with inappropriate neurons are formed. The physiological properties of the regenerated synaptic connections (correct and incorrect) will be determined. If correct synaptic connections are found, I will determine if they are stable or subsequently lost as the specific connections are established. The results will help elucidate the mechanisms by which central neurons regenerate and form specific synaptic contacts. I will also examine the intraganglionic connections of the same neurons in each ganglion for plastic changes in synaptic function during the regeneration process. If plastic changes are observed., I will determine the physiological and morphological bases for those changes. I will also determine if the functioning of the synapses returns to normal when the interganglionic synaptic connections are reestablished. This will provide insights into how neurons regulate their synaptic connections.

我研究的长期目标是了解如何了解 神经元相互认识并形成特定的突触连接。 这 拟议的研究将检查化学的特异性和可塑性 大脑和 Aplysia的胸膜神经节。 神经元具有特定的单突触 每个神经节内的连接（内部连接）。并与 另一个神经节中的神经元（gan膜间连接）。 每个突触 连接是一种生理独特的类型：快速EPSP，慢速EPSP，IPSP。 神经元在轴切开后通过压碎而再生 脑棕褐色缔合。 这破坏了gan膜间突触 连接，但没有内部连接。 两者中的神经元 神经节将使用细胞内记录和 通过细胞内染料注射在形态上， 轴突再生之后。 我将确定是否再生神经元 仅与其特定关注者或如果 与不适当的神经元的突触形成。 生理 再生突触连接的特性（正确和不正确） 将确定。 如果找到正确的突触连接，我将 确定它们是稳定还是随后丢失为特定 建立了联系。 结果将有助于阐明 中央神经元再生并形成特定突触的机制 联系人。 我还将检查相同的内部连接 每个神经节中的神经元，用于在 再生过程。 如果观察到塑料变化。 确定这些变化的生理和形态基础。 我 还将确定突触的功能是否返回正常 当重新建立gan膜间突触连接时。 这会 提供有关神经元如何调节其突触连接的见解。