PATHOLOGY OF CELL DIFFERENTIATION IN LEUKEMIA

白血病细胞分化的病理学

• 批准号: 3163911
• 负责人: DOROTHY F BAINTON
• 金额: $ 9.73万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-06-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3163911
• 关键词: acute monocytic leukemia; blood proteins; bone marrow; carcinogenesis; cell differentiation; cellular oncology; electron microscopy; enzyme structure; granule; hematopoiesis; histochemistry /cytochemistry; human subject; immunochemistry; leukemia; leukocyte disorder; leukocytes; monocyte; myelogenous leukemia; neutrophil; newborn human (0-6 weeks); organelles; pediatric neoplasm /cancer; phagocytosis; radiotracer; tissue /cell culture

The goal is to study the abnormal differentiation of leukocytes and to establish better methods for the diagnosis and classification of leukemias and lymphomas. Recently, using a modification of our plastic embedding technique, we analyzed the distribution of nine enzymes (5'-NT, ATP, alkaline and acid phosphatase, beta-glucuronidase, alpha-NAE, alpha-NBE, CAE, and peroxidase) in human lymphoid tissue and clearly showed the ability of this technique to accurately identify macrophages in these tissues. Furthermore, several stromal cells can be labeled (e.g., the fibroblastic reticulum cells of the paracortex with alkaline phosphatase and the dendritic reticulum cell with 5'NT). Using this technique, anaplastic seminomas and large cell lymphomas may be easily distinguished in that the cells of the germ cell malignancy are outlined with alkaline phosphatase. We are currently expanding the number of distinguishing cell markers on plastic embedded pathologic biopsy material by applying immunocytochemical methods. We have developed a well-defined phenotype for definition of Histiocytosis X(ANAE+, AcP+, AcP+, Ia+, OKT6+) and demonstrated its identity with human Langerhans cells. Using a similar approach we have shown the Kaposi's sarcoma is mainly an unusual proliferation of lymphatic endothelium. (4)

目的是研究白细胞的异常分化并 建立更好的白血病诊断和分类方法 和淋巴瘤。 最近，通过改进我们的塑料嵌入技术，我们 分析了九种酶（5'-NT、ATP、碱性和酸性 磷酸酶、β-葡萄糖醛酸酶、α-NAE、α-NBE、CAE 和过氧化物酶） 在人体淋巴组织中，清楚地显示了该技术的能力 准确识别这些组织中的巨噬细胞。 此外，几个 基质细胞可以被标记（例如，成纤维细胞网状细胞） 副皮质具有碱性磷酸酶，树突状网状细胞具有 5'NT）。 使用这种技术，间变性精原细胞瘤和大细胞淋巴瘤 很容易区分生殖细胞恶性肿瘤的细胞 用碱性磷酸酶勾勒出轮廓。 我们目前正在扩大 塑料包埋病理活检中区分细胞标记物的数量 通过应用免疫细胞化学方法制备材料。 我们开发了一个 用于定义组织细胞增多症 X 的明确表型（ANAE+、AcP+、AcP+、 Ia+、OKT6+）并证明了其与人类朗格汉斯细胞的同一性。 使用类似的方法，我们已经证明卡波西肉瘤主要是 淋巴管内皮异常增殖。 (4)