BIOENERGETIC REGULATION AND NEUROLOGICAL MATURATION

生物能量调节和神经成熟

• 批准号: 3399473
• 负责人: MERLE S OLSON
• 金额: $ 9.31万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-04-01 至 1986-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3399473
• 关键词: bioenergetics; growth /development; hormone regulation /control mechanism; infant animal; ketone body; mitochondria; phosphorylation; pyruvate dehydrogenase; radiotracer; synaptosomes

The research described in this application is designed to characterize the regulatory properties of the pyruvate dehydrogenase multienzyme complex in the rat brain. Metabolic flux through the pyruvate dehydrogenase reaction, the activation state of the enzyme complex and the level of phosphorylation of the enzyme will be determined in mitochondrial and synaptosomal preparations derived from several individual regions of the brain taken from adult rats and from rats during their early developmental stages. The regulation of pyruvate dehydrogenase or pyruvate metabolism in general will be investigated in perfused brains derived from adult rats and developing rats. The effects of alterations in the hormal and/or the nutritional state of the animal on the regulatory parameters for the pyruvate dehydrogenase complex will be investigated in this metabolic preparation. The long range goal of this research project is to document the crucial relationship between the ability ofthe brain to regulate its important energy generating pathways and the maintenance of neuro-logical competence in this tissue.

本应用程序中描述的研究旨在表征 丙酮酸脱氢酶多酶复合物的调节性能 大鼠大脑。 丙酮酸脱氢酶反应的代谢通量， 酶复合物的激活状态和磷酸化水平 酶的酶将在线粒体和突触体中确定 从大脑的几个单个区域得出的制剂 从成年大鼠和大鼠的早期发育阶段。 这 调节丙酮酸脱氢酶或丙酮酸代谢的调节将会 在源自成年大鼠并发育的灌注大脑中进行调查 老鼠。 荷正和/或营养的改变的影响 丙酮酸的调节参数上动物的状态 将在这种代谢制剂中研究脱氢酶复合物。 该研究项目的远距离目标是记录关键 大脑调节其重要的能力之间的关系 能源产生途径和神经逻辑能力的维持 在这个组织中。

项目成果

Fluorocarbon perfusion of the isolated rat brain: measurement of tissue spaces, EEG and oxygen uptake.

离体大鼠脑的氟碳灌注：组织空间、脑电图和摄氧量的测量。

• DOI: 10.1016/0304-4165(91)90220-b
• 发表时间: 1991
• 期刊: Biochimica et biophysica acta
• 作者: Harvey,SA;Trankina,ML;Olson,MS;Clark,JB
• 通讯作者: Clark,JB