MEMBRANE PROPERTIES OF NEOCORTICAL NEURONS

新皮质神经元的膜特性

• 批准号: 3397123
• 负责人: WAYNE E. CRILL
• 金额: $ 13.42万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-04-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3397123
• 关键词: acetylcholine; aminoacid; brain cell; central nervous system; cholinergic agents; electrophysiology; membrane permeability; membrane structure; motor cortex; neural information processing; neurons; sensory cortex; synapses

We request funds to continue our in vitro studies of the electrophysiological properties of single large neurons in layer V of the cat pericruciate neocortex. In the last decade and particularly in the last five years major advances have been made in our understanding of the ionic mechanisms of the responses of neurons in the mammalian central nervous system (CNS). Our long-term objective is to understand the mechanisms whereby neurons of the mammalian CNS transduce synaptic inputs into spike trains for transmission of information on to the next cellular relay. In these experiments we will use the slice preparation and either the single or double microelectrode voltage clamp to measure ionic currents. In addition to studying voltage- and time-dependent currents, we will examine the effects of putative neurotransmitters such as acetylcholine, excitatory amino acids and neuropeptides that, based on the studies of others, cause a transient change (modulation) in the ionic currents and therefore the transduction properties of these neurons. These studies have health and biologic significance. The large neurons of layer V of sensorimotor cortex are the major output to lower centers and the spinal cord. If we are to understand major neurological problems such as epilepsy and degenerative diseases of cortex such as amyotrophic lateral sclerosis and Alzheimer's disease we must first know the normal neurobiologic properties of neocortical neurons.

我们要求资金继续我们的体外研究 单个大型神经元在V层V层的电生理特性 猫核酸内新皮层。 在过去的十年中，尤其是 过去五年来，我们对 哺乳动物中心神经元反应的离子机制 神经系统（CNS）。 我们的长期目标是了解 哺乳动物CNS的神经元转导突触输入的机制 进入尖峰火车以传输到下一个蜂窝的信息 中继。 在这些实验中，我们将使用切片准备，要么 单个或双微电极电压夹子以测量离子 电流。 除了研究电压和时间相关电流外，我们还 将检查假定的神经递质的影响，例如 乙酰胆碱，兴奋性氨基酸和神经肽，这些基于 对他人的研究，在离子中引起瞬时变化（调制） 电流，因此这些神经元的转导特性。 这些 研究具有健康和生物学意义。 层的大神经元 感觉运动皮层的V是较低中心的主要输出 脊髓。 如果我们要了解主要的神经系统问题，例如 皮质的癫痫和退化性疾病，例如肌萎缩性侧面 硬化症和阿尔茨海默氏病我们必须首先知道正常 新皮质神经元的神经生物学特性。