STUDIES ON MAMMALIAN NEURONS AND GLIA IN VITRO

哺乳动物神经元和神经胶质细胞的体外研究

• 批准号: 3396362
• 负责人: BRUCE Robert RANSOM
• 金额: $ 21.77万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3396362
• 关键词: acid base balance; astrocytes; cerebral cortex; electrophysiology; extracellular; glia; homeostasis; membrane permeability; neuroanatomy; neurochemistry; neurons; neuropharmacology; neurophysiology; neurotransmitters; slow potential; acid base balance; astrocytes; cerebral cortex; electrophysiology; extracellular; glia; homeostasis; membrane permeability; neuroanatomy; neurochemistry; neurons; neuropharmacology; neurophysiology; neurotransmitters; slow potential

The proposed research represents a continuation of studies on some of the basic physiological, morphological, and pharmacological properties of mammalian glia and neurons in in vitro systems. This research will also investigate the characteristics and homeostatic regulation of ionic and volume fluctuations in the extracellular space (ECS) of in vitro preparations. Some of these issues will be carefully studied at different stages during postnatal development and correlated with important morphological or biochemical changes occurring at these times. A new research direction for this plan is the analysis of the single ion channel in cultured or isolated glial cells. Isolated rat optic nerves, tissue slices from rat neocortex, and cultured rodent astrocytes will be used in most experiments and offer several advantages over in vivo preparations including ready access of perfusion solutions to the ECS, direct visualization of microanatomic features of the tissue cells, and relative ease of intracellular recordings from neurons and glia. One component of the project will investigate characteristics of some of these issues in a non-mammalian preparation, the turtle brain. Using intracellular staining and recording techniques the following projects will be undertaken: 1) developmental study of glial cell physiology and morphology in the rat optic nerve; 2) electrophysiological, morphological, and metabolic studies on glia in the rat optic nerve; 3) mechanisms of slow potential generation and ECS ionic homeostasis in turtle cortex; 4) characterization of neural activity-dependent changes of pHo in rat optic nerve and neocortical tissue slice; 5) mechanisms of neural activity dependent ECS shrinkage; 6) patch clamp studies on single ionic channels of glia in vitro.

拟议的研究代表了一些有关某些研究的研究 基本的生理，形态和药理特性 体外系统中的哺乳动物神经元和神经元。 这项研究也将 研究离子和的特征和稳态调节 体外细胞外空间（EC）的体积波动 准备。 其中一些问题将在不同的 产后发展期间的阶段，并与重要 在这些时候发生形态学或生化变化。 一个新 该计划的研究方向是对单个离子通道的分析 在培养或孤立的神经胶质细胞中。 孤立的大鼠视神经，组织 大鼠新皮层的切片和培养的啮齿动物星形胶质细胞将用于 大多数实验并提供了与体内准备相比的几个优势 包括准备灌注解决方案到ECS，直接 可视化组织细胞的微解剖特征和相对 易于神经元和神经胶质的细胞内记录。 一个组成部分 该项目将调查其中一些问题的特征 非哺乳动物制剂，乌龟大脑。 使用细胞内染色 和记录技术将进行以下项目：1） 大鼠神经胶质细胞生理学和形态的发展研究 视神经； 2）电生理，形态学和代谢研究 大鼠视神经中的神经胶质； 3）潜在生成缓慢的机制 和ECS离子稳态在海龟皮质中； 4）神经的表征 大鼠视神经和新皮质组织中PHO的活性依赖性变化 片; 5）神经活动依赖EC的机制收缩； 6）补丁 夹紧在体外对神经胶质的单离子通道进行研究。