OLIGOCLONAL IGG IN CHRONIC RELAPSING EAE

慢性复发性 EAE 中的寡克隆 IGG

• 批准号: 3395524
• 负责人: PANKAJ D. MEHTA
• 金额: $ 5.85万
• 依托单位: INSTITUTE FOR BASIC RES IN DEV DISABIL
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-04-01 至 1987-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3395524
• 关键词: antibody formation; bacterial antigens; blood brain barrier; brain; central nervous system; disease /disorder model; electrofocusing; experimental allergic encephalomyelitis; gel electrophoresis; genetic strain; humoral immunity; immunochemistry; immunoglobulin G; model design /development; multiple sclerosis; relapse /recurrence; remission /regression; serum globulins; spectrometry; spinal cord

The sensitization of young strain 13 guinea pigs with homologous spinal cord in complete Freund's adjuvant and killed mycobacterium tuberculosis has been shown to result in a relapsing and remitting disease in about 65% of the animals injected. Our recent results have shown that the IgG was significantly increased in CSF of animals sacrificed during the first remission, first relapse and late stages of the disease. The isoelectric focusing patterns of CSF and serum from relapsing EAE animals, showed oligoclonal IgG bands. Since these results are consistent with those observed in multiple sclerosis, we propose to study the specificity of the oligoclonal IgG using animals with chronic R-EAE and those injected with streptococcal antigen. Although the latter group did not develop EAE, their CSF showed presence of oligoclonal IgG bands. The specificity of the oligoclonal IgG to specific brain and bacterial antigens in the above animals will be determined by imprint electroimmunofixation method. It is anticipated that these studies will lead to a better understanding of the synthesis of oligoclonal band formation in multiple sclerosis.

同源脊髓幼年品系13豚鼠致敏 弗氏完全佐剂的脊髓并杀死结核分枝杆菌 已证明约 65% 的患者会导致疾病复发和缓解 注射动物的数量。 我们最近的结果表明 IgG 第一次处死动物的脑脊液显着增加 缓解期、首次复发和疾病晚期。 等电点 复发性 EAE 动物的脑脊液和血清的聚焦模式显示 寡克隆 IgG 带。 由于这些结果与那些一致 在多发性硬化症中观察到，我们建议研究其特异性 使用患有慢性 R-EAE 的动物和注射了 链球菌抗原。 尽管后者没有出现EAE， 他们的脑脊液显示存在寡克隆 IgG 带。 的特殊性 针对上述特定脑和细菌抗原的寡克隆 IgG 动物将通过印迹电免疫固定法进行测定。 这是 预计这些研究将有助于人们更好地理解 多发性硬化症中寡克隆带形成的合成。

项目成果

Pathogenetic aspects of multiple sclerosis and experimental models of inflammatory demyelination.

多发性硬化症的发病机制和炎性脱髓鞘的实验模型。

• 发表时间: 1985
• 期刊: Concepts in immunopathology
• 作者: Wisniewski,HM;Schuller-Levis,GB;Mehta,PD;Madrid,RE;Lassmann,H
• 通讯作者: Lassmann,H

Oligoclonal IgG band patterns in chronic relapsing EAE and MS.

慢性复发性 EAE 和 MS 中的寡克隆 IgG 带模式。

• 发表时间: 1984
• 期刊: Progress in clinical and biological research
• 作者: Mehta,PD;Patrick,BA;Wisniewski,HM
• 通讯作者: Wisniewski,HM

Specificity of oligoclonal IgG bands in sera from chronic relapsing experimental allergic encephalomyelitis guinea pigs.

慢性复发实验性过敏性脑脊髓炎豚鼠血清中寡克隆 IgG 带的特异性。

• 发表时间: 1985
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Mehta,PD;Patrick,BA;Wisniewski,HM
• 通讯作者: Wisniewski,HM

Immunologic studies of chronic relapsing EAE in guinea pigs: similarities to multiple sclerosis.

豚鼠慢性复发性 EAE 的免疫学研究：与多发性硬化症的相似之处。

• 发表时间: 1981
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Mehta,PD;Lassmann,H;Wisniewski,HM
• 通讯作者: Wisniewski,HM

Chronic relapsing EAE in guinea pigs: IgG index and oligoclonal bands in cerebrospinal fluid and sera.

豚鼠慢性复发性 EAE：脑脊液和血清中的 IgG 指数和寡克隆带。

• DOI: 10.3109/08820138509022670
• 发表时间: 1985
• 期刊: Immunological investigations
• 影响因子: 2.8
• 作者: Mehta,PD;Patrick,BA;Mehta,SP;Wisniewski,HM
• 通讯作者: Wisniewski,HM