MOLECULAR INTERACTIONS BETWEEN EBV & HIV

EBV 之间的分子相互作用

• 批准号: 3138733
• 负责人: MARIE-LOUISE HAMMARSKJOLD
• 金额: $ 13.72万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3138733
• 关键词: B lymphocyte; Burkitt's lymphoma; Epstein Barr virus; genetic manipulation; helper T lymphocyte; human immunodeficiency virus 1; human tissue; latent virus infection; lymphoma; tissue /cell culture; virus genetics; virus replication; virus virus interaction

Epstein-Barr Virus/(EBV) is emerging as one of the most important co-factors in AIDS/ARC. Reactivation of EBV is common in AIDS and AIDS patients frequently develop EBV- associated lymphomas. In addition, it has recently been shown that HIV can replicate in B-cells transformed by EBV, but not in EBV-negative B-cells. In this proposal the molecular interactions between EBV and HIV will be studied. Using the EBV-negative Burkitt Lymphoma lines Ramos and BJAB and several EBV converted counterparts of these cell lines, the replication of HIV and the expression of transfected HIV genes will be analysed. Cell lines expressing individual latent EBV proteins (EBNA1, EBNA2, EBNA3, LMP) will be created and analysed for their ability to support HIV replication. Binding and fusion experiments will be performed to determine if the receptor used for the uptake of HIV into B-cells is T4 or some other entity. The effect of HIV-infection on EBV-expression in latently infected cells will also be analyzed. In addition lymphoma cells from AIDS-patients will be characterized to determine how many of these lymphomas that are EBV-positive. The EBV-positive lymphomas will be analyzed for association with specific substrains of EBV and for restricted expression of the latent EBV- genes.

Epstein-Barr 病毒/(EBV) 正在成为最常见的病毒之一 AIDS/ARC 的重要辅助因素。 EBV 的重新激活是 常见于 AIDS 且 AIDS 患者经常感染 EBV- 相关淋巴瘤。 另外，最近还曝光了 HIV可以在EBV转化的B细胞中复制，但不能在 EBV 阴性 B 细胞。 在这个提议中，分子相互作用 将研究 EBV 和 HIV 之间的关系。 使用 EBV 阴性伯基特淋巴瘤系 Ramos 和 BJAB 以及这些细胞系的几种 EBV 转化对应物， HIV的复制和转染的HIV基因的表达 将被分析。 表达个体潜伏 EBV 的细胞系 蛋白质（EBNA1、EBNA2、EBNA3、LMP）将被创建并 分析了它们支持 HIV 复制的能力。 绑定和 将进行融合实验以确定受体是否 用于将 HIV 摄取到 B 细胞中的是 T4 或其他一些实体。 HIV感染对EBV潜伏表达的影响 受感染的细胞也将被分析。 另外还有淋巴瘤细胞 来自艾滋病患者的特征将被确定，以确定有多少 这些淋巴瘤呈 EBV 阳性。 EBV阳性 将分析淋巴瘤与特定的关联 EBV 亚株和潜在 EBV- 的限制性表达 基因。