RED CELL PRESERVATION

红细胞保存

• 批准号: 3363687
• 负责人: TIBOR J GREENWALT
• 金额: $ 14.53万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3363687
• 关键词: adenosine triphosphate; ammonium compounds; blood banks; blood donor; blood preservation; cell age; erythrocytes; human subject; phosphates; solutions

The long term goal is to extend the storage shelf life of human red cells (RBC) with improved in vivo survival. An additive-preservative solution containing ammonium (NH4+) and phosphate (Pi) ions in addition to adenine, glucose, and mannitol has been shown to maintain adenosine triphosphate (ATP) levels of RBC high enough for more than 12 weeks at 4 to suggest good in vivo RBC survivals. The aims are to find the survival studies to establish the maximum storage interval compatible with acceptable posttransfusion survival; to perform metabolic studies to establish the biochemical pathways involved in the maintenance of high ATP concentrations under these conditions. Metabolic studies are designed to determine whether increased synthesis of AMP or its decreased degradation of both occur. The role of phosphofructokinase activity will also be explored. Preliminary data indicate a strong possibility that satisfactory storage of RBC for 12 or more weeks can be achieved. The resulting blood donor programs; conservation of the blood supply by decreased loss through outdating; and decreasing the usage of blood and the rate of tissue iron accumulation in chronically transfused patients because of the longer in vivo survival of better preserved RBC.

长期目标是延长人类红细胞的存储货架寿命 （RBC）改善了体内存活率。 添加剂保存解决方案 除腺嘌呤外，还含有铵（NH4+）和磷酸盐（PI）离子 葡萄糖和甘露醇已显示可维持三磷酸腺苷 （ATP）RBC的水平足够高12周以上，以表明 良好的体内RBC存活。 目的是找到生存研究 建立与可接受的最大存储间隔兼容 转移后生存；进行代谢研究以建立 维持高ATP浓度涉及的生化途径 在这些条件下。 代谢研究旨在确定 是否增加了AMP的合成或两者的降低降解 发生。 还将探讨磷酸果果因酶活性的作用。 初步数据表明很有可能令人满意的存储 可以实现RBC 12个或更长时间。 由此产生的献血者 程序；通过减少损失通过 过时；并降低血液的使用和组织铁的速率 由于长期较长的患者积累 保存更好的RBC的体内生存。