LASER IRRADIATION AND VASCULAR REACTIVITY

激光照射和血管反应性

• 批准号: 3357728
• 负责人: Jeffrey Isner
• 金额: $ 17.31万
• 依托单位: ST. ELIZABETH'S MEDICAL CENTER OF BOSTON
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3357728
• 关键词: analgesics; argon; atherosclerosis; cimetidine; diltiazem; drug metabolism; guinea pigs; indomethacin; ketamine; laboratory rabbit; laser therapy; particle beam; phentolamine; prazosin; propranolol; pulse pressure wave; pulse radiolysis; radiation therapy; radiopharmacology; vascular smooth muscle; vasomotion

Laser energy, employed as a focused or transmitted via optical fibers, has been demonstrated in vitro, in vivo in animal models, and more recently in vivo in human patients to be capable of ablating atherosclerotic lesions responsible for stenosis or total occlusion of peripheral and coronary arteries. Although the interaction between laser light and the atherosclerotic lesion has been investigated in some detail, the effect of laser light on vascular smooth muscle has not been previously studied. Accordingly, the aims of this proposal are as follows: 1) to determine the effect of continuous wave (CW) laser- irradiation on vascular smooth muscle; 2) to determine the effect of pulsed laser irradiation on vascular smooth muscle; 3) to investigate the extent to which either the effects of CW or pulsed laser irradiation on vascular smooth muscle are wavelength-dependent; 4) to determine the relationship between laser-induced vasomotor reactivity and thermal behavior of the irradiated segment of vascular smooth muscle; 5) to establish the relationship between the vector and magnitude of laser-induced vasomotor reactivity and the functional and anatomic status of the endothelial layer of the irradiated segment of vascular smooth muscle; and 6) to determine the extent to which laser-induced vasomotion can be modified by various pharmacologic agonists and antagonists. To accomplish these specific aims, a series of in vitro and in vivo experiments will be performed using CW (argon, Nd:YAG) and pulsed (excimer, tuneable dye) lasers. For in vitro experiments, laser irradiation will be delivered to isolated ring segments of aorta obtained from healthy and atherosclerotic New Zealand white rabbits and mounted isometrically in Krebs bicarbonate buffer. For in vivo experiments, laser irradiation will be delivered percutaneously via optical fibers in normal New Zealand white rabbits and rabbits and microswine with atherosclerotic lesions induced by an atherogenic diet and balloon-endothelial denudation. From a fundamental standpoint, the experiments described in this proposal will establish the range of vascular smooth muscle responses to laser irradiation and provide insight regarding the responsible mechanisms. From a practical standpoint, these experiments may have important implications regarding the choice of lasers and the manner in which such lasers are employed for clinical purposes.

激光能量，用于通过光学的聚焦或传播 纤维在体外已被证明，在动物模型中，体内 最近，人类患者的体内有能力 消融负责狭窄或总的动脉粥样硬化病变 周围和冠状动脉的阻塞。 虽然 激光光与动脉粥样硬化病变之间的相互作用具有 已详细研究了激光对 先前尚未研究血管平滑肌。 因此，该提议的目的如下： 1）确定连续波（CW）激光的影响 血管平滑肌的照射； 2）确定脉冲激光照射对血管的影响 平滑肌; 3）研究CW或CW的影响的程度 血管平滑肌上的脉冲激光照射是 波长依赖性； 4）确定激光诱导的血管舒缩剂之间的关系 辐照段的反应性和热行为 血管平滑肌； 5）建立向量与幅度之间的关系 激光诱导的血管舒缩反应性和功能和功能 辐照段的内皮层的解剖状态 血管平滑肌；和 6）确定激光诱导的血管症的程度 由各种药理激动剂和拮抗剂修饰。 为了实现这些特定目标，一系列体外和体内 实验将使用CW（Argon，ND：YAG）和 脉冲（准分子，可调染料）激光器。 对于体外实验， 激光照射将被传递到孤立的环段 从健康和动脉粥样硬化的新西兰获得的主动脉 白兔子，在碳酸氢盐的克雷布斯 缓冲。 对于体内实验，激光照射将是 通过正常新西兰的光纤经术传递 白兔子，兔子和微酮与动脉粥样硬化 动脉粥样硬化饮食和气球 - 内皮引起的病变 剥夺。 从根本的角度来看，实验中描述了 建议将建立一系列血管平滑肌 对激光照射的反应，并提供有关 负责任的机制。 从实际的角度来看 实验可能对 激光的选择和使用这种激光器的方式 出于临床目的。