DIETARY MFA AND LIPOPROTEIN STRUCTURE AND FUNCTION

膳食 MFA 和脂蛋白的结构和功能

• 批准号: 3356094
• 负责人: SHLOMO EISENBERG
• 金额: $ 15.58万
• 依托单位: HADASSAH-HEBREW UNIVERSITY MEDICAL CTR
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3356094
• 关键词: apolipoproteins; atherosclerosis; binding proteins; blood coagulation tests; blood lipid; blood lipoprotein; blood lipoprotein metabolism; blood tests; cell membrane; cholesterol; coronary disorder; diet therapy; dietary carbohydrates; dietary fiber; dietary lipid; disease /disorder prevention /control; disease /disorder proneness /risk; electron microscopy; erythrocyte membrane; essential fatty acids; fibroblasts; gas chromatography; gel electrophoresis; hemostasis; high density lipoproteins; human subject; hyperlipidemia; immunologic techniques; lipid metabolism; lipid structure; lipid transport; longitudinal human study; low density lipoprotein; monocyte; nutrition related tag; peroxidation; phospholipids; platelets; radiotracer; receptor; saturated fatty acids; statistics /biometry; thin layer chromatography; tissue /cell culture; tocopherols; triglycerides; ultracentrifugation; unsaturated fatty acids; vascular endothelium; vascular smooth muscle; very low density lipoprotein

Almost all major epidemiological and intervention studies have incriminated elevated levels of blood cholesterol and LDL-C as significant risk factors for coronary morbidity and mortality, while HDL-C has a protective role. Attempts to reduce the risk for coronary heart disease have emphasized the amount of total fat, saturated (SFA) and polyunsaturated (PFA) fatty acids and cholesterol in the diet. An effect of dietary monounsaturated fatty acids (MFA) - hitherto believed to have no influence on blood lipids - has been recently proposed. However, it has not been definitely demonstrated that this effect of dietary MFA was due to enrichment with MFA specifically, or the reduction in dietary SFA, increase of the P:S ratio or other secondary changes in the diet composition. We propose to investigate the specific effect of dietary MFA on lipoprotein structure and function in a free-living population of young health Israeli males (aged 18-24 yr), living on a college (yeshiva) campus. Five different experiments will be performed to compare the effects of a diet with a high MFA content and one with a low MFA content, as follows: 1) High MFA - low PFA vs. Low MFA - high PFA; 2) High MFA vs. Low MFA - high starch; 3) High MFA vs. Low MFA - high SFA 4) High MFA vs. Low MFA; 5) High MFA vs. Low MFA - low fat. The amounts of cholesterol, sugar and fiber will be similar in all diets. Each subject will consume two of the diets for a 3-month period in a cross-over design study. The specific objectives are to study the influence of dietary MFA on blood lipids, lipoprotein classes and subfractions, their structure and composition, their uptake by specific receptors, their degradation by cultured fibroblasts, and their reverse transport in plasma. Changes in the fatty acid content of cells and lipoproteins will be followed and their susceptibility to peroxide formation measured. In addition, the effect of dietary MFA on non-lipid parameters of atherogenesis such as platelet function will also be evaluated.

几乎所有主要的流行病学和干预研究都有 血液胆固醇和LDL-C水平升高为 冠状动脉发病率和死亡率的重要危险因素， 而HDL-C具有保护作用。 试图降低风险 对于冠状动脉疾病已经强调了总数 脂肪，饱和（SFA）和多不饱和（PFA）脂肪酸和 饮食中的胆固醇。 饮食单不饱和的效果 脂肪酸（MFA） - 迄今据信对 血脂 - 最近提出。 但是，没有 肯定证明了饮食MFA的这种影响是 由于专门用MFA富集或减少 饮食SFA，P：S比或其他次要变化的增加 在饮食组成中。 我们建议研究特定 饮食MFA对脂蛋白结构和功能的影响 以色列男性的年轻健康卫生人口（18-24岁） YR），住在大学（Yeshiva）校园里。 五个不同 将进行实验以比较饮食的影响 MFA含量高，MFA含量低，AS 以下内容： 1）高MFA-低PFA与低MFA-高PFA； 2）高MFA与低MFA-高淀粉； 3）高MFA与低MFA -高SFA 4）高MFA与低MFA； 5）高MFA与低MFA-低脂。 胆固醇，糖和纤维的量在所有人中都相似 饮食。 每个受试者将三个月消耗两种饮食 跨界设计研究的时期。 具体目标是 研究饮食MFA对血脂，脂蛋白的影响 班级和子分数，它们的结构和组成，它们 特定受体的摄取，培养的降解 成纤维细胞及其在等离子体中的反向运输。 变化 将遵循细胞和脂蛋白的脂肪酸含量，并且 它们对过氧化物形成的敏感性进行了测量。 此外， 饮食MFA对非脂质参数的影响 还将评估诸如血小板功能之类的动脉粥样硬化。