CELLS PROCESSING HIGH DENSITY LIPOPROTEINS

处理高密度脂蛋白的细胞

• 批准号: 3346195
• 负责人: EVE P REAVEN
• 金额: $ 9.84万
• 依托单位: FRIENDS RESEARCH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-04-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3346195
• 关键词: adrenal glands; autoradiography; blood lipoprotein metabolism; cholesterol; cholesterol esters; corpus luteum; electron microscopy; freeze etching; high density lipoproteins; liver metabolism; nonelectrolyte transport; pinocytosis; radiotracer; receptor mediated endocytosis; steroid biosynthesis; steroid metabolism; thin layer chromatography; tissue /cell culture; tritium

The proposed studies will provide an in-depth ultrastructural-biochemical examination of the process by which high density lipoproteins (HDL) deliver cholesterol to mammalian cells. Specifically, we hope to identify which cells in a given organ are responsible for HDL uptake, and which aspects of the cells themselves (surface membrane sites or intracellular organelles) are involved in HDL-uptake events. Although the majority of our structurally-oriented experiments are, of necessity, directed toward tracking the protein moiety of HDL in cells, each experiment will be linked to a parallel biochemical experiment in which the relevance of a given structural event involving HDL-protein is evaluated in terms of the cell's utilization of HDL-delivered cholesterol. In addition to these efforts, we plan to test specific morphological techniques which may allow us, in the future, to directly track cholesterol in cells. In large part, we have proposed follow-up experiments on information obtained in an earlier study using the luteinized ovary as a tissue model. The planned studies can be divided into 4 major parts as follows: 1) experiments designed to retest our observation that ovarian luteal cells in vivo do not internalize HDL-protein as part of an HDL-initiated steroidogenic response; 2) experiments designed to test the observation that ovarian cells in vivo may process HDL differently than ovarian cells in vitro; 3) experiments designed to enlarge these questions regarding HDL processing to include another steroidogenic organ (adrenal) and a non-steroidogenic organ (liver); and 4) efforts to develop morphological techniques which would permit us to track the delivery of cholesterol, itself, in tissues.

拟议的研究将提供深入的超微结构生物化学 检查高密度脂蛋白（HDL）传递的过程 胆固醇与哺乳动物细胞。 具体来说，我们希望确定哪个 给定器官中的细胞负责HDL吸收，以及 细胞本身（表面膜位点或细胞内细胞器） 参与HDL摄取事件。 虽然我们的大多数 以结构为导向的实验是针对的 跟踪细胞中HDL的蛋白质部分，每个实验都将连接 进行平行的生化实验，其中给定的相关性 根据细胞的评估，评估涉及HDL蛋白的结构事件 利用HDL降低的胆固醇。 除了这些努力，我们 计划测试特定的形态技术，这可能使我们在 未来，直接跟踪细胞中的胆固醇。 在很大程度上，我们提出了有关信息的后续实验 在较早的研究中，使用叶酸卵巢作为组织模型获得。 计划的研究可以分为4个主要部分：1） 旨在重新观察到卵巢黄体细胞中的实验 体内不要将HDL蛋白内化为HDL发射的一部分 类固醇反应； 2）旨在测试观察的实验 体内卵巢细胞的处理可能与卵巢细胞不同。 体外; 3）旨在扩大有关HDL的这些问题的实验 加工以包括另一个类固醇器官（肾上腺）和A 非替代器官（肝脏）； 4）努力发展形态学 使我们能够跟踪胆固醇的传递的技术， 本身，在组织中。