VASCULAR ENDOTHELIUM AND FLUID SHEAR STRESS IN VITRO

体外血管内皮和流体剪切应力

• 批准号: 3338119
• 负责人: C Forbes Dewey
• 金额: $ 30.63万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-04-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3338119
• 关键词: artificial intelligence; atherosclerosis; autoradiography; blood lipoprotein metabolism; cell adhesion; cell migration; cinemicrography; clone cells; cytoskeleton; eicosanoid metabolism; embryo /fetus tissue /cell culture; hemodynamics; human tissue; mathematical model; mechanical stress; peptidyl dipeptidase A; pinocytosis; radiotracer; regeneration; scanning electron microscopy; silver impregnation; vascular endothelium; wound healing

The structural and functional alterations of vascular endothelium caused by fluid shear stress will be investigated in vitro using cultured endothelial cells and a special apparatus we have developed for producing controlled fluid shear stress. The apparatus utilizes a shallow cone rotating above a flat plate to produce shear stresses between 10 to the -2 and 200 dynes/cm2; both laminar and turbulent flow conditions can be checked. Additional modifications will provide oscillating and alternating shear stress levels to investigate the response of the cells to shear conditions approximating those existing in vivo. Specific parameters of endothelial structure and function to be measured include: growth patterns and growth kinetics; migration and mitosis following injury; cell orientation and morphology; rates and mechanisms of endocytosis; cytoskeletal organization; and interaction with extracellular matrix. This is a collaborative research effort that draws upon the resources, expertise, and experience of the Fluid Mechanics Laboratory, Massachusetts Institute of Technology and the Vascular Pathophysiology Laboratory, Brigham and Women's Hospital. The research system to be used in this program, comprised of an accurately controlled fluid shear stress apparatus and well defined cultured endothelial specimens, should yield valuable information on the influence of fluid shear stress on the structure and function of vascular endothelium. Such information would provide new insights into vascular physiology and the pathogenesis of vascular diseases such as atherosclerosis.

由 液体剪切应力将在体外使用培养的内皮研究 我们开发了用于产生受控的细胞和一种特殊的设备 流体剪切应力。 该设备利用一个浅锥，在上方旋转 扁平板产生剪切应力在-2至200之间 Dynes/CM2;可以检查层状和湍流条件。 其他修改将提供振荡和交替的剪切 压力水平以研究细胞对剪切条件的反应 近似那些现有的体内。 内皮的特定参数 结构和功能要衡量包括：增长模式和增长 动力学;受伤后的迁移和有丝分裂；细胞取向和 形态学;内吞作用的速率和机制；细胞骨架组织； 并与细胞外基质相互作用。 这是一个协作 借鉴资源，专业知识和经验的研究工作 流体力学实验室，马萨诸塞州技术研究所和 血管病理生理学实验室，杨百翰和妇女医院。 这 该程序中使用的研究系统由准确的 受控的流体剪切应力设备和良好的培养 内皮标本应产生有关影响的有价值的信息 流体剪切应力在血管的结构和功能上 内皮。 这些信息将为血管提供新的见解 生理学和血管疾病的发病机理，例如 动脉粥样硬化。