MULTIPLE CARDIAC INPUTS TO ASCENDING PATHWAYS

多种心脏输入上升通道

• 批准号: 3337042
• 负责人: ROBERT D FOREMAN
• 金额: $ 14.73万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-12-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3337042
• 关键词: Macaca mulatta; afferent nerve; analgesia; angina pectoris; antidromic impulse; brain mapping; brain stem; cardiovascular pharmacology; cats; electrophysiology; electrostimulus; heart pharmacology; microelectrodes; myocardial ischemia /hypoxia; neuroanatomy; pain; reticular formation; somesthetic sensory cortex; spinal nerves; thalamus; vagus nerve

Cardiovascular adjustments and pain sensation usually occur when an organism encounters a "painful stimulus" associated with ischemia and infarction of the heart, but sometimes the insult does not produce pain (i.e. the "silent infarct"). The intermediate and long term objectives are to record activity of cells of origin of the spinoreticular tract (SRT) and the medial and lateral spinothalamic tract (STT) system, of the thalamus, and of the somatosensory cortex and examine the complex interactions that occur between the cardiovascular system, particularly the heart, and the pain system. The working hypothesis of this proposal is that activation of vagal afferent fibers stimulates brainstem structures whose projections descend to modulate the activity of SRT and STT neurons in the T1-T5 segments of the spinal cord. The upper thoracic spinal cord of cats (SRT cells) and monkey (STT cells) will be explored with carbon filament or tungsten microelectrodes to record single extracellular action potentials of cells antidromically activated from the thalamus or the medullary reticular formation. The specific aims are to: 1) determine effects of electrically, mechanically or chemically stimulating vagal afferent fibers on activity of SRT and STT cells responding to visceral and somatic inputs; 2) determine nuclei and descending pathways activated during vagal stimulation by applying focal electrical and chemical stimulation and by making lesions; 3) simultaneously record activity of raphe-spinal and STT and SRT cells during vagal stimulation; and 4) examine the effects of independently occluding branches of the left coronary artery and the right coronary artery on the activity of STT or SRT cells before and after vagal cooling or vagal stimulation. This study will contribute to understanding the interactions of neural mechanisms that underlie cardiac pain, the associated angina pectoris and possibly the "silent infarction".

心血管调节和疼痛感通常发生在以下情况： 有机体遇到与缺血相关的“痛苦刺激” 心脏梗塞，但有时侮辱不会产生疼痛 （即“无声梗塞”）。 中期和长期目标是 记录脊髓网状束 (SRT) 起源细胞的活动 丘脑的内侧和外侧脊髓丘脑束（STT）系统， 和体感皮层并检查复杂的相互作用 发生在心血管系统，特别是心脏和 疼痛系统。 该提案的工作假设是激活 迷走神经传入纤维刺激脑干结构，其投射 下降调节 T1-T5 中 SRT 和 STT 神经元的活动 脊髓的节段。 猫的上胸段脊髓（SRT 细胞）和猴子（STT细胞）将用碳丝或 钨微电极记录单个细胞外动作电位 从丘脑或髓质逆向激活的细胞 网状结构。 具体目标是： 1) 确定效果 电、机械或化学刺激迷走神经传入纤维 SRT 和 STT 细胞响应内脏和躯体输入的活动； 2) 确定迷走神经期间激活的细胞核和下行通路 通过应用局部电刺激和化学刺激以及通过 造成病变； 3) 同时记录中缝脊髓和STT的活动 迷走神经刺激期间的 SRT 细胞； 4）检查效果 独立闭塞左冠状动脉和右冠状动脉的分支 冠状动脉对迷走神经前后STT或SRT细胞活性的影响 冷却或迷走神经刺激。 这项研究将有助于理解 心脏疼痛背后的神经机制的相互作用 相关的心绞痛和可能的“无症状梗塞”。