NPY BIOSYNTHESIS AND SECRETION IN THE HYPOTHALAMUS

下丘脑中的 NPY 生物合成和分泌

• 批准号: 3328413
• 负责人: JOHN K MC DONALD
• 金额: $ 15.68万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3328413
• 关键词: arachidonate; dopamine; estrogens; female; hormone regulation /control mechanism; hypothalamic pituitary axis; hypothalamus; laboratory rat; luteinizing hormone; median eminence; microdialysis; microinjections; neuroendocrine system; neuropeptide Y; norepinephrine; ovariectomy; peptide hormone biosynthesis; perfusion; posttranslational modifications; protein kinase C; radioimmunoassay; radionuclide double label; reproductive hormone; secretion; sex hormones

Objectives: This proposal is designed to examine the neuroendocrine control of the synthesis and release of neuropeptide Y (NPY) in the hypothalamus of female rats following alterations in plasma levels of gonadal steroids. The proposed research should enhance our basic understanding of the reproductive neuroendocrine regulation of NPY biosynthesis and secretion which is part of our long-term objective to elucidate the function of NPY in the neuroendocrine events which precede ovulation. This knowledge may provide new insight into the fundamental neuroendocrine mechanisms which control reproduction and fertility and thereby facilitate our understanding of pathological changes which accompany infertility. Aims: 1. To examine the regulation of NPY secretion from the hypothalamus using in vivo and in vitro techniques. The role of LHRH, gonadal steroids, catecholamines, and two potential intracellular mechanisms involved in catecholaminergic modulation of NPY release will be explored using short-term cultures of medial basal hypothalamic fragments, push-pull perfusion and microdialysis of the medial basal hypothalamus, microdialysis of the anterior pituitary gland, and collection of pituitary portal blood. 2. To investigate the hypothesis that gonadal steroids regulate NPY biosynthesis in the basal hypothalamus of female rats. The effects of altered levels of gonadal steroids on NPY biosynthesis will be determined in ovariectomized rats treated with estrogen, estrogen and progesterone, or vehicle. These rats will receive injections of [3H] tyrosine into the third cerebral ventricle to examine, in vivo, the gonadal steroid dependency of the biosynthesis and posttranslational processing of NPY in the arcuate nucleus.

目标：该建议旨在检查神经内分泌 控制神经肽Y（NPY）的合成和释放 血浆水平改变后，雌性大鼠的下丘脑 性腺类固醇。 拟议的研究应增强我们的基本 了解NPY的生殖神经内分泌调节 生物合成和分泌是我们长期目标的一部分 阐明NPY在之前的神经内分泌事件中的功能 排卵。 这些知识可能会提供有关基本的新见解 控制繁殖和生育能力的神经内分泌机制以及 从而有助于我们对病理变化的理解 伴随不育症。 目标： 1。检查下丘脑的NPY分泌的调节 使用体内和体外技术。 LHRH，性腺类固醇的作用， 儿茶酚胺和两种潜在的细胞内机制 NPY释放的Catecholamin能调制将使用 内侧下丘脑碎片的短期培养物，推扣 内侧下丘脑，微透析的灌注和微透析 垂体前腺和垂体血液的收集。 2。研究性腺类固醇调节NPY的假设 雌性大鼠基底下丘脑的生物合成。 效果 将确定NPY生物合成上性腺类固醇水平的改变 在用雌激素，雌激素和孕激素治疗的卵巢切除大鼠中， 或车辆。 这些大鼠将接受[3H]酪氨酸的注射 第三个脑室，在体内检查性腺类固醇 NPY在生物合成和翻译后的依赖性 弧形核。