SOCIAL AND PHYSIOLOGICAL REGULATION OF MATURATION

成熟的社会和生理调节

• 批准号: 3324079
• 负责人: RUSSELL D FERNALD
• 金额: $ 16.91万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1994-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3324079
• 关键词: alternatives to animals in research; animal developmental psychology; electrophoresis; fertility; fish; fresh water environment; gene expression; gonadotropin releasing factor; histology; hormone regulation /control mechanism; in situ hybridization; messenger RNA; neural information processing; neural plasticity; neuroendocrine system; nucleic acid probes; olfactions; prosencephalon; radioassay; sex; testosterone; touch; vision; western blottings

The research proposed here addresses internal and external regulators of sexual maturation in vertebrates. Specifically, development of forebrain magnocellular neurons immunoreactive to gonadotropon-releasing hormone (irGnRH) are to be studied in a teleost using cellular and molecular probes. Forebrain irGnRH neurons comprise a phylogenetically ancient system which plays a central role in modulating pituitary control of gonadal maturation in all vertebrate classes. While analysis of this system in mammals is complicated by the superimposition of neocortical functions, basal forebrain organization can be readily studied in teleosts. The irGnRh neurons and their modulation of pituitary function have important consequences to the organism and are often implicated in growth or maturation related disease (hypogonadism and juvenilized physiolgnomy). In the teleost, H. burtoni, both development of irGnRH neurons as measured by some size and complexity of neurite morphology, and gonadal maturation are influenced by social interactions. Competition form larger conspecifics suppresses maturation in young adult fish, causing both undersized irGnRH forebrain neurons and hypogonadism. Since we can control the social system, this system is highly advantageous for analysis of maturation. Two classes of experiments are proposed: One to identify the sensory modality which is responsible for mediating social signals which suppress maturation and the second to ablate two key systems, olfactory bulb and gonads, implicated in forebrain maturation. We will measure the consequences of these experiments with immunoreactivity to GnRH antibody and molecular probes to the mRNA of GnRH. We will thus be able to discover the cellular and molecular consequences of social regulation of maturation. Since the results of social interaction are quite extreme. This may set the stage for understanding a pathway for the social regulation of gene action.

这里提出的研究涉及 脊椎动物的性成熟。 具体而言，开发 前脑大细胞神经元对促性腺释放的免疫反应性 激素（IRGNRH）将在使用细胞和 分子探针。 前脑IRGNRH神经元包括系统发育 古代系统在调节垂体中起着核心作用 控制所有脊椎动物类别的性腺成熟。 分析 哺乳动物中的该系统的叠加使 新皮层功能，基础前脑组织可以很容易地 在Telests学习。 IRGNRH神经元及其调节 垂体功能对生物有重要影响，并且是 通常与生长或成熟相关疾病有关 和少年的生理术）。 在Teolost中 通过神经突的大小和复杂性和性腺的复杂性来衡量 成熟受社会互动的影响。 竞争表格 较大的种子抑制成年鱼的成熟，导致 两者尺寸不足的IRGNRH前脑神经元和性腺功能减退。 既然我们可以 控制社会系统，该系统对 成熟分析。 提出了两类实验：一个识别感觉的实验 导致调解社会信号的方式 抑制成熟，第二次消融两个关键系统：嗅觉 鳞茎和性腺，涉及前脑的成熟。 我们将通过 对GnRH抗体和分子探针的免疫反应性 gnrh。因此，我们将能够发现细胞和分子 社会调节成熟的后果。 由于结果 社交互动非常极端。 这可能为 了解基因作用社会调节的途径。