REGULATION OF THE PRIMATE CORPUS LUTEUM

灵长类黄体的调节

• 批准号: 3325827
• 负责人: FIRYAL S KHAN-DAWOOD
• 金额: $ 9.89万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3325827
• 关键词: autoradiography; cell membrane; chorionic gonadotropin; corpus luteum; eicosanoid metabolism; embryo implantation; estradiol; estrone; high performance liquid chromatography; hormone receptor; hormone regulation /control mechanism; menstrual cycle; ovary; oxytocin; progesterone; prostaglandin F; radioimmunoassay; steroid biosynthesis; tissue /cell culture; uterus; vasopressins

The corpus luteum (CL) plays a major role in the primate reproductive process. The hormones produced by the CL are necessary for the successful implantation of the ovum in the uterus and hence a successful pregnancy. Little is known about the mechanisms involved in the control of this "endocrine" gland. Recent evidence indicates that several gonadal peptides one of which is oxytocin (OT) may be involved in luteal development. Using the baboon as an experimental model the objectives of this study are a) to evaluate the effects of prostaglandin F2a on CL hormone production, b) to examine the effect of OT and hCG on PGF, 6- ketoprostaglandin F1a and 5-HETE production. These effects will be examined using an in vitro dispersed cell preparation, in short term incubations (3 hr), long term (24-48 hr) culture and in vivo using local infusion techniques, c) to examine the exact site in the steroidogenic pathway where OT may have its effect, d) to determine if the action of OT is receptor mediated, using CL membrane preparations and autoradiography, e) to measure PGF 2a receptors using CL membrane preparation and f) to determine if ovarian OT is biologically active using an in vitro bioassay. The hormones in the media from the in vitro studies and in blood (femoral ovarian veins, contralateral to the CL and ipsilateral vein) will be assayed by radioimmunoassay. Enzyme assays will be used to determine the site of action of OT in corpus luteum steroidogenesis. The data obtained will provide a more integrated picture of the mechanisms controlling the primate CL which may permit new approaches to fertility regulation as well as a better understanding of therapy of early reproductive failures or difficulties due to ovulatory or corpus luteum dysfunction. Similarly it may enable new approaches to the treatment of menstrual dysfunction.

黄体（CL）在灵长类动物生殖中发挥着重要作用 过程。 CL 产生的激素对于成功来说是必要的 将卵子植入子宫，从而成功怀孕。 人们对控制这种现象的机制知之甚少。 “内分泌”腺。最近的证据表明，一些性腺 肽其中之一是催产素（OT）可能与黄体有关 发展。使用狒狒作为实验模型的目的 本研究的目的是 a) 评估前列腺素 F2a 对 CL 的影响 激素产生，b) 检查 OT 和 hCG 对 PGF 的影响，6- 酮前列腺素 F1a 和 5-HETE 的产生。这些影响将是 在短期内使用体外分散细胞制剂进行检查 孵化（3小时）、长期（24-48小时）培养和体内使用局部 输注技术，c) 检查类固醇生成的确切位点 OT 可能发挥作用的途径，d) 确定 OT 的作用是否 是受体介导的，使用 CL 膜制剂和放射自显影， e) 使用 CL 膜制剂测量 PGF 2a 受体，f) 使用体外测定确定卵巢 OT 是否具有生物活性 生物测定。体外研究和体内培养基中的激素 血液（股卵巢静脉、CL 对侧和同侧 静脉）将通过放射免疫测定法进行测定。酶测定将用于 确定 OT 在黄体类固醇生成中的作用位点。这 获得的数据将提供更全面的机制图景 控制灵长类动物的 CL 可能会带来新的生育方法 监管以及对早期治疗的更好理解 由于排卵或黄体导致的生殖失败或困难 功能障碍。同样，它可能会带来新的治疗方法 月经失调。