INVESTIGATING HORMONAL REGULATION OF SPERMATOGENESIS

研究精子发生的激素调节

基本信息

批准号：
3315315
负责人：
ROGER C COCHRAN
金额：
$ 6.38万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-07-01 至 1987-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3315315
关键词：
Leydig cells Sertoli cells androgens autoradiography cell cell interaction germ cells hormone metabolism hormone regulation /control mechanism meiosis radioimmunoassay radiotracer seminal vesicles sperm spermatogenesis synchronous cell division temperature tissue /cell culture

项目摘要

The development of an in vitro system for investigating the hormonal regulation of spermatogenesis is proposed. Endocrinological similarities in the control of spermatogenesis in mammals and fish permit analogies to be drawn regarding hormonal regulation of the processes. It is hypothesized that the anatomical and physiological characteristics of Fundulus heteroclitus (the common killifish) testes will enable germinal cells in testicular fragments to be stimulated to undergo meiosis and/or spermiogenesis in vitro, thus providing a simple model for mammalian spermatogenesis. The testicular recrudescence of Fundulus, reproductively synchronized by manipulation of temperature and photoperiod in the laboratory, will be examined. Gonadotropin levels in the pituitaries of fish with regressed and recrudesced testes will be determined. Qualitative and quantitative changes in testicular androgen metabolism, in vivo, will be correlated with morphometric analyses of the percentages of different spermatogenic stages of germinal cysts appearing in testes at discrete time points during the process of recrudescence. The optimum temperature for culturing Fundulus testes will be determined by measuring the length of time required for labeled thymidine to appear in spermatozoa at different temperatures in vivo. In vitro, testicular fragments containing germinal cysts of differing spermatogenic stages from reproductively synchronized animals will then be cultured to see if: 1) spermatid cysts will poduce spermatozoa, 2) spermatocyte cysts will produce spermatids, and 3) spermatogonial cysts will produce spermatocytes. The attainment of these objectives will determine the basic endocrinological changes associated with the anatomical alterations occuring during spermatogenesis in Fundulus testes, establish which stages of spermatogenesis are androgen dependent, and create a defined system for examining the hormonal regulation of the interactions of germinal cells with other testicular cells.

用于研究激素的体外系统的开发提出了精子发生的调节。内分泌相似性在控制哺乳动物和鱼类的精子发生时，类比允许类比在过程的荷尔蒙调节方面被绘制。这是假设的解剖学和生理特征地眼杂片（普通杀伤）睾丸将使生发睾丸片段中的细胞被刺激以进行减数分裂和/或体外精子发生，因此为哺乳动物提供了简单的模型精子发生。地眼睛的睾丸复发，通过生殖同步实验室中的温度和光周期操纵将是检查。鱼垂体中的促性腺激素水平退化并将确定被重塑的睾丸。定性和定量体内睾丸雄激素代谢的变化将与不同精子阶段百分比的形态分析在离散时间点出现在睾丸中的生发囊肿复发过程。培养原则的最佳温度睾丸将通过测量所需的时间长度来确定标记为胸苷，在不同温度下出现在精子中体内。体外，睾丸碎片，含有含有生发囊肿的睾丸生殖同步动物的精子阶段不同然后将培养以查看是否：1）精子囊肿会导致精子，2）精子囊肿将产生精子，3）精子囊肿将产生精子细胞。实现这些目标将决定基本与解剖学改变相关的内分泌学变化在白大睾丸中的精子发生过程中发生，确定哪个阶段精子发生是雄激素依赖性的，并为检查生发细胞相互作用的激素调节与其他睾丸细胞。