VITAMIN A IN GROWTH AND DEVELOPMENT

生长和发育中的维生素 A

• 批准号: 3311058
• 负责人: FRANK CHYTIL
• 金额: $ 2.59万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3311058
• 关键词: RNA; all trans retinol; analytical method; autoradiography; binding proteins; cell differentiation; cell nucleus; developmental nutrition; electron microscopy; gene expression; genetic transcription; glucocorticoids; growth /development; histochemistry /cytochemistry; immunochemistry; intestinal mucosa; laboratory rat; liver; lung; mature animal; molecular pathology; newborn animals; nucleic acid hybridization; nucleoproteins; nutrition related tag; postnatal growth disorder; protein sequence; radioimmunoassay; radiotracer; receptor; retinoate; testis; tissue /cell culture; vitamin A deficiency; vitamin metabolism

The objective of proposed in vivo as well as in vitro nutritional and biochemical research is to continue to define the mechanisms(s) of action of the important micronutrient vitamin A at the molecular level in organs other than the eye. The participation in the mode of vitamin action of two distinct specific intracellular cytoplasmic binding proteins for retionl (vitamin A alcohol) and for retinoic acid (vitamin A acid) purified to homogeneity from rat liver and testis will receive particular attention. In vitro studies are proposed how retinol and retinoic acid bound to their respective binding proteins interact with the cell nucleus changing thereby the genomic expression underlying cell differentiation. The in vivo studies are directed towards the elucidation of factor which regulate the levels of these binding proteins in various organs in adult and in neonatal rats. To this end sensitive radioimmunoassys developed for both binding proteins will be utilized.

拟议的体内和体外营养和 生化研究将继续定义作用机制 器官分子水平的重要微量营养素A的 除了眼睛。 参与两种维生素作用模式 重度的不同特异性细胞内细胞质结合蛋白 （维生素A酒精）和视黄酸（维生素A酸）纯化为 大鼠肝脏和睾丸的同质性将受到特别关注。 提出了视黄醇和视黄酸如何与其结合的体外研究 各自的结合蛋白与细胞核相互作用，因此 基因组表达的基础细胞分化。 体内 研究是针对调节因素的阐明 成人和新生儿中各种器官中这些结合蛋白的水平 老鼠。 为此，为这两种结合而开发了敏感的放射免疫群 蛋白质将被利用。