REGULATION OF HIV MRNA 3' PROCESSING

HIV mRNA 3 加工的调控

• 批准号: 3306056
• 负责人: GREGORY M GILMARTIN
• 金额: $ 13.8万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3306056
• 关键词: Escherichia coli; RNA binding protein; chimeric proteins; genetic regulation; human immunodeficiency virus; messenger RNA; molecular cloning; polyadenylate; posttranscriptional RNA processing; protein structure function

DESCRIPTION: (Adapted from applicant's abstract) The central goal of this project is to try to elucidate the biochemical basis of the regulation of HIV mRNA 3' processing. The long-term objective is to exploit the unique nature of the HIV poly(A) site processing signal to design an effective antiviral agent. The investigator proposes to identify the factor responsible for recognition of the HIV poly(A) site upstream element and the mechanism by which it enhances processing will be delineated.The subsequent cloning of this upstream factor and the characterization of its functional domains will permit the alteration of the RNA-binding specificity of the factor in order to facilitate recognition and subsequent processing of the HIV 5' core poly(A) site. A well-characterized in vitro processing system will be employed to probe the biochemical basis of the regulation of HIV mRNA 3' end formation. The investigator has five specific aims the first of which is to try to identify the processing factor that interacts with the HIV poly(A) site upstream element. Next, the author proposes to elucidate the mechanism by which the upstream factor functions to enhance processing at the HIV poly(A) site. Third, he will attempt to purify biochemically the HIV poly(A) site upstream factor and to isolate the corresponding cDNA clones. He also proposes to identify the domains of the upstream factor that mediate sequence recognition and function to enhance processing. Lastly, the author will try to develop a chimeric processing factor, and potential antiviral agent, to facilitate processing at the HIV 5' core poly(A) site.

描述：（改编自申请人的摘要） 项目是试图阐明调节的生化基础 HIV mRNA 3'处理。 长期目标是利用独特 HIV Poly（A）站点处理信号的性质以设计有效 抗病毒剂。 研究人员建议确定因素 负责识别HIV Poly（a）上游元素的艾滋病毒（A）和 将描述增强处理的机制。 随后的上游因子的克隆及其表征 功能域将允许改变RNA结合 该因素的特异性以促进识别和随后的识别 HIV 5'核心poly（a）位点的处理。 体外良好的体外 处理系统将被用来探测其生化基础 HIV mRNA 3'末端形成的调节。 调查员有五个 具体目的第一个是尝试识别处理 与HIV Poly（A）站点上游元素相互作用的因素。 下一个， 作者建议阐明上游因素的机制 在HIV Poly（A）位点增强加工的功能。 第三，他会的 尝试在生化上纯化HIV Poly（a）位点上游因素，然后 分离相应的cDNA克隆。他还建议确定 介导序列识别和 功能以增强处理。 最后，作者将尝试开发 嵌合处理因子和潜在抗病毒剂，以促进 在HIV 5'Core Poly（A）位点进行处理。