ENERGY TRANSDUCTION BETWEEN MEMBRANES

膜间的能量转换

• 批准号: 3300693
• 负责人: KATHLEEN POSTLE
• 金额: $ 14.04万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3300693
• 关键词: Escherichia coli; active transport; bacterial proteins; bioenergetics; cytoplasm; gel electrophoresis; genetic manipulation; genetic transcription; immunoprecipitation; membrane permeability; membrane proteins; membrane transport proteins; microorganism metabolism; mutant; posttranslational modifications; protein structure function; protein transport; siderophores; stoichiometry; vitamin B12

The goal of the proposed research is to understand biological energy transduction between membranes using the Gram-negative bacterium E. coli as the model system. In E. coli, cytoplasmic membrane energy is coupled to the active transport of important nutrients (iron-bearing sideropheres and vitamin B12) across the outer membrane. The mechanism of energy transduction remains to be elucidated. Several proteins have been shown genetically to participate in the phenomenon, most likely in the form of energy transduction complexes. Emphasizing TonB protein, the protein most directly involved in energy transduction, we will attempt to characterize the structure, function, and regulation of energy transduction complexes. The specific aims of this project are 1.) to determine the composition and stoichiometries of TonB-dependent energy transduction complexes. 2.) to elucidate the role of chemical modifications in TonB-dependent energy transduction. 3.) to delineate the mechanism of coupling between the cytoplasmic membrane proton-motive force and the energy-requiring processes of the outer membrane. 4.) to characterize the mechanisms of transcriptional regulation of TonB. Research in this area will have important ramifications for understanding energy transduction, protein-protein interactions, and membrane phenomena in general.

拟议的研究的目的是了解生物学 使用革兰氏阴性细菌之间的膜之间的能量转导 大肠杆菌作为模型系统。 在大肠杆菌中，细胞质膜能为 与重要营养物质的主动运输相结合（含铁 跨膜的媒介物和维生素B12）。 机制 能量转导仍有待阐明。 几种蛋白质具有 从遗传上显示以参与该现象，很可能 能量转导复合物的形式。 强调tonb蛋白， 蛋白质最直接参与能量转导，我们将尝试 表征能量的结构，功能和调节 转导复合物。 该项目的具体目的是1.） 确定依赖TONB的能量的组成和化学计量法 转导复合物。 2.）阐明化学的作用 TONB依赖性能量转导的修改。 3.）描绘 胞质膜质子 - 动物之间的耦合机制 力和外膜的能量征收过程。 4.）到 表征TONB转录调控的机制。 该领域的研究将对理解有重要的影响 能量转导，蛋白质 - 蛋白质相互作用和膜现象 一般来说。