CHARACTERIZATION OF ARABIDOPSIS TELOMERES

拟南芥端粒的表征

• 批准号: 3302570
• 负责人: ERIC JEAN RICHARDS
• 金额: $ 5万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1992-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3302570
• 关键词: Arabidopsis; DNA; DNA replication; chemical structure function; chromosome disorders; chromosomes; eukaryote; gel electrophoresis; genetic mapping; genome; molecular cloning; mutant; nucleic acid probes; nucleic acid repetitive sequence; plant genetics; telomere; trisomy

This proposal is a part of a long-term project to understand the organization of higher eukaryotic genomes and the molecular biology of functional chromosome components, such as telomeres and centromeres. Telomeres are the specialized structures that form the termini of eukaryotic chromosomes. These structures are essential for the replication and maintenance of the genome. Telomeres have been extensively studied in lower eukaryotes but considerably less in known about higher eukaryotic telomeres. We have recently isolated a higher eukaryotic telomere from the flowering plant Arabidopsis thaliana, providing the opportunity to study the telomeric regions of this organism as a model system. Two aspects of telomere structure and function will be studied. First, the nature and organization of the DNA sequences that lie adjacent to A. thaliana telomeres will be investigated. Second, the mechanism by which a broken chromosome end acquires a new telomere (i.e., telomere healing) will be studied using a chromosomal mutant of A. thaliana which carries a healed telomere. The healed telomere will be isolated and its structure compared to that of the original chromosomal site to determine how the healing event took place. In addition, the healed telomere, which resides proximal to a centromere, will be investigated as a potential tool for studying A. thaliana centromere structure.

该建议是一个长期项目的一部分，以了解 高等真核基因组的组织和分子生物学 功能性染色体成分，例如端粒和丝粒。 端粒是形成末端的专业结构 真核生物染色体。 这些结构对于复制至关重要 和基因组的维护。 端粒已被广泛研究 较低的真核生物，但对较高的真核知识已知要少得多 端粒。 我们最近将较高的真核端粒与 开花植物拟南芥，提供了学习的机会 该生物作为模型系统的端粒区域。 将研究端粒结构和功能的两个方面。 首先， 与A相邻的DNA序列的自然和组织。 Thaliana端粒将进行调查。 第二，一种机制 破碎的染色体末端获得了新的端粒（即端粒愈合）将 使用A. thaliana的染色体突变体研究 治愈端粒。 愈合的端粒将被隔离及其结构 与原始染色体部位相比 康复事件发生了。 此外，居住的端粒 靠近着丝粒，将作为潜在的工具进行研究 研究A. thaliana Centromere结构。