MITOCHONDRIA SHARE ENZYMES WITH THE REST OF THE CELL

线粒体与细胞的其他部分共享酶

• 批准号: 3301013
• 负责人: Nancy C Martin
• 金额: $ 17.54万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1994-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3301013
• 关键词: Saccharomyces cerevisiae; biological signal transduction; cell components; cell nucleus; cytoplasm; enzyme biosynthesis; eukaryote; gene mutation; immunofluorescence technique; immunoprecipitation; mitochondria; molecular cloning; protein biosynthesis; protein transport; radionuclides; transfer RNA; transferase

Proteins synthesized in the cytoplasm must be sorted to other parts of the eukaryotic cell to function. Most have a single destination but there is a unique subset of proteins which must be partitioned between two cellular compartments. The TRM1 and MOD5 gene products are tRNA modification enzymes that are dually localized in the yeast Saccharomyces cerevisiae. The TRM1 gene product is the only known example of a gene that codes for proteins destined to the mitochondria and nucleus while the MOD5 product is mitochondrial and cytoplasmic, not nuclear. The objective of the proposed studies is to understand the mechanisms that result in the dual localization of these two gene products. First, we will determine how the enzymes found in the different compartments are synthesized from the genes encoding them. Second, molecular biological and cell biological methods will be used to determine what portions of the proteins contain signals necessary to target them to the mitochondria and the nucleus and whether the absence of these signals leads to cytoplasmic accumulation. Third, we will determine how a protein with both mitochondrial and nuclear targeting signals can be localized exclusively to mitochondria. Fourth, we will use genetic approaches to address the questions of why proteins are sequestered to certain organelles. Finally, we will attempt to identify genes encoding soluble factors and membrane receptors that comprise the cellular machinery essential for proper localization of MOD5 and TRM1 proteins.

在细胞质中合成的蛋白质必须分类为其他部分 真核细胞的功能。 大多数有一个目的地 但是有一个独特的蛋白质子集必须被分割 在两个细胞室之间。 TRM1和MOD5基因产品 是tRNA修饰酶，这些酶在 酿酒酵母的酵母糖酵母。 TRM1基因产物是唯一的 代码为蛋白质的基因的已知示例 线粒体和核，而Mod5产物是线粒体 和细胞质，而不是核。 提议的目标 研究是了解导致双重的机制 这两个基因产物的定位。 首先，我们将确定 如何合成不同隔室中发现的酶 从编码它们的基因。 第二，分子生物学和 细胞生物学方法将用于确定哪些部分 蛋白质包含针对它们的信号 线粒体和细胞核以及是否没有这些 信号导致细胞质积累。 第三，我们会的 确定线粒体和核的蛋白质如何 靶向信号可以专门定位于线粒体。 第四，我们将使用遗传方法来解决 为什么将蛋白质隔离为某些细胞器。 最后，我们 将尝试识别编码可溶性因素和的基因 包括细胞机械必需的膜受体 用于正确定位MOD5和TRM1蛋白。