NATURE OF AUDITORY NERVE REGENERATION

听觉神经再生的本质

• 批准号: 3080318
• 负责人: GEORGE T HASHISAKI
• 金额: $ 8.79万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-15 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3080318
• 关键词: acoustic nerve; auditory nuclei; auditory pathways; brain stem; cell differentiation; chick embryo; cochlea; developmental neurobiology; electrophysiology; embryo /fetus tissue /cell culture; fluorescent dye /probe; gerbil /jird; laboratory mouse; labyrinthine membrane; mixed tissue /cell culture; nervous system regeneration; neural inhibition; neural initiation; neural plasticity; neural transmission; neuroeffector; neurofilament; neuronal guidance; newborn animals; organ of Corti; phase contrast microscopy; single cell analysis; synaptogenesis

The long term goal of this project is to maintain a cell culture of the inner ear, the auditory nerve, and the auditory nuclei of the brain stem. These structures represent the first echelon of the auditory nervous system and such a model would mark a significant contribution to studies on the auditory system. The myriad physiologic and pharmacologic manipulations which could be performed on such an in vitro system could rapidly expand our knowledge of the auditory system. Using aseptic techniques of surgery and cell culture the inner ear membranous tissue, the eight nerve fibers, and the auditory nuclei of the brain stem will be harvested from three animal groups--chicken embryos, neonatal gerbils, and neonatal mice.These tissues will be explanted onto cover slips pretreated with collagen and will be incubated with culture media.The living tissue will be examined during maintenance with phase--contrast microscopy. Tissue treated with fixatives will also be examined with fluorescent markers of nerve fibers. The morphology of the cultured tissue will be evaluated. Electrophysiologic recordings from the cultured tissue will be obtained. With the thin monolayer configuration of the cultured tissue, it will be possible to clearly identify the cells from which electrical signals are recorded. This is a unique opportunity to examine multiple sites along the auditory pathway in a continuous manner.

该项目的长期目标是维持细胞培养 内耳、听觉神经和脑干的听觉核团。 这些结构代表听觉神经系统的第一梯队 这样的模型将对相关研究做出重大贡献 听觉系统。 无数的生理和药理操作 可以在这样的体外系统上进行，可以迅速扩展 我们对听觉系统的了解。 使用无菌手术技术和细胞培养内耳 膜组织、八根神经纤维和听觉核团 脑干将从三个动物群体中获取——鸡胚胎、 新生沙鼠和新生小鼠。这些组织将被移植到 用胶原蛋白预处理的盖玻片并将与培养物一起孵育 介质。在维护期间将检查活体组织 相差显微镜。 用固定剂处理过的组织也将被 用神经纤维的荧光标记进行检查。 的形态 将评估培养的组织。 培养组织的电生理记录将 获得。 由于培养组织的薄单层结构， 将有可能清楚地识别产生电的细胞 信号被记录。 这是一个独特的机会来检查多个 沿听觉通路连续分布的位置。