DOLICHOL METABOLISM AND MEMBRANE GLYCOPROTEIN BIOSYNTHES

多醇代谢和膜糖蛋白生物合成

• 批准号: 3289674
• 负责人: Charles J Waechter
• 金额: $ 15.3万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-06-01 至 1987-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3289674
• 关键词: animal age group; brain; brain metabolism; carbohydrate biosynthesis; carbohydrate structure; central nervous system; dolichol; embryo /fetus; enzyme structure; glycolipids; glycosyltransferase; growth /development; heparan sulfate; lipase; lipid metabolism; microsomes; neurochemistry; neurotransmitter metabolism; peptidases; phosphoric ester; radiotracer; sulfotransferase; tissue /cell culture

Studies with calf brain microsomes and tissue slices on the den novo synthesis of dolichyl monophosphate are proposed. Extensive work on the purification of dolichol kinase, dolichyl monophosphatase and dolichyl ester lipase will be done to establish their properties and specificity. The possibility that changes in these activities influence the level of dolichyl monophosphate during development in the central nervous system will be explored. In addition the subcellular location(s) of the three enzymes mentioned above and the various lipid-mediated glycosyltransferases in brain will be studied. We shall also attempt to purify sulfotransferases involved in the biosynthesis of a glycoprotein and proteoheparan sulfate associated with calf brain membranes. The structural requirements and the properties of the binding interaction between the proteoheparan sulfate and calf membranes will be investigated.

在den novo上使用小腿脑微粒体和组织切片的研究 提出了多磷酸二磷酸的合成。 关于 多利果醇激酶，多甲基单磷酸酶和多乙基的纯化 酯脂肪酶将进行建立其特性和特异性。 这些活动变化的可能性会影响 中枢神经系统发育过程中的多磷酸二磷酸 将被探索。 另外，三个的亚细胞位置 上面提到的酶和各种脂质介导的糖基转移酶 将研究大脑。 我们还将尝试净化 参与糖蛋白和 与小牛脑膜相关的硫酸盐硫酸盐。 结构 要求和结合相互作用的特性 将研究硫酸盐蛋白酶和小牛膜。