PACKAGING OF THE SEGMENTED GENOME OF BACTERIOPHAGE O6

噬菌体 O6 分段基因组的包装

• 批准号: 3285201
• 负责人: LEONARD E MINDICH
• 金额: $ 16.94万
• 依托单位: PUBLIC HEALTH RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-01-01 至 1989-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3285201
• 关键词: Escherichia coli; Pseudomonas; bacterial virus; chromosomes; complementary DNA; double stranded RNA; gene expression; genetic manipulation; genetic mapping; genetic transcription; genome; molecular cloning; nucleic acid sequence; virus envelope; virus genetics; virus protein

Bacteriophage Phi6 contains three pieces of double-stranded RNA in its nucleocapsid. The pieces contain different genetic information but have similar termini. The aim of this proposal is to determine how the packaging system is able to select one of each of the chromosomes. Specifically, the regions of the three chromosomes necessary for packaging will be identified. The order of packaging and the requirements for packaging in terms of amount of RNA will be determined. The studies will depend upon the use of cDNA clones of the Phi6 chromosomes. Chimeras and other constructions of the cDNA clones will be prepared and the packaging of the transcripts of the cDNA will be studied. In addition, cDNA clones of the large chromosome will be prepared. The products of this chromosome should form procapsids capable of packaging RNA. The nature of the mechanisms of selective packaging in viruses of segmented genomes is currently unknown. The elucidation of the mechanism for Phi6 will probably be relevant to packaging of other segmented genome viruses.

噬菌体 Phi6 的内部含有三段双链 RNA 核衣壳。 这些片段包含不同的遗传信息，但具有 类似的终点站。 本提案的目的是确定如何 包装系统能够选择每条染色体之一。 具体来说，包装所需的三个染色体区域 将被识别。 包装顺序及要求 包装的RNA量将被确定。 这些研究将取决于 Phi6 cDNA 克隆的使用 染色体。 嵌合体和 cDNA 克隆的其他构建体将被 将研究 cDNA 转录物的制备和包装。 此外，还将制备大染色体的cDNA克隆。 这 该染色体的产物应形成能够包装 RNA 的原衣壳。 分段病毒选择性包装机制的本质 基因组目前未知。 Phi6机制的阐明 可能与其他分段基因组病毒的包装有关。