A STUDY OF DDC, AND A SCREEN FOR NEW LEARNING MUTANTS

DDC 研究以及新学习突变体的筛选

• 批准号: 3287087
• 负责人: WILLIAM G QUINN
• 金额: $ 10.26万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-09-01 至 1986-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3287087
• 关键词: aromatic L aminoacid decarboxylase; association learning; behavioral habituation /sensitization; conditioning; dopamine; gene expression; gene mutation; growth media; high performance liquid chromatography; learning; molecular psychobiology; mutagen testing; neurotransmitter metabolism; neurotransmitters; olfactions; serotonin; temperature sensitive mutant

We are looking for insights into the mechanisms underlying learning and memory storage. The approach we use is to isolate single-gene mutations that block learning or current memory. Then, using a variety of techniques, we compare our mutations with their parent strains in a search for differences relevant to learning. We have developed a new olfactory discriminative avoidance conditioning procedure that produces a dramatic improvement in learning levels; flies show a 90% probability to make a correct choice. We propose here to use this new conditioning procedure two ways. First, working with ddc mutations (dopa decarboxylase deficient; produces no measurable dopamine or serotonin synthesis and does not learn), we will attempt to revert the learning deficiency by feeding flies monoamine transmitters or their precursors. With this type of procedure we hope to determine which neurotransmitter is principally involved with learning. Second, we propose to screen for and isolate new learning mutations more efficiently. Currently, three of six learning mutants have been characterized biochemically. To pursue our approach further, we need new learning mutants. The strong learning obtained with the new training procedure should enable us to "enrich" the F1 progeny from mutagenized males before screening individual homozygous lines, greatly increasing our efficiency in isolating new mutants.

我们正在寻找有关学习机制和 内存存储。 我们使用的方法是隔离单基因突变 那个阻止学习或当前记忆。 然后，使用各种 技术，我们将突变与他们的父母菌株进行比较 与学习相关的差异。 我们已经开发了一个新的嗅觉 产生戏剧性的判别避免调节程序 学习水平的提高；苍蝇显示出90％的概率 正确的选择。 我们在这里建议使用此新调节过程两个 方式。 首先，使用DDC突变（DOPA脱羧酶缺乏； 不产生可测量的多巴胺或5-羟色胺合成，不学习）， 我们将尝试通过喂食苍蝇来恢复学习不足 单胺发射器或其前体。 使用这种类型的过程，我们 希望确定哪种神经递质主要涉及 学习。 其次，我们建议筛选并隔离新学习 突变更有效。 目前，六个学习突变体中有三个有 在生化上被表征。 为了进一步追求我们的方法，我们需要 新的学习突变体。 通过新培训获得的强大学习 程序应该使我们能够从诱变中“富集” F1后代 在筛选单个纯合线之前的雄性，大大增加了我们的 隔离新突变体的效率。