GENETIC ANALYSIS OF THE BASAL BODY CYCLE

基础身体周期的遗传分析

• 批准号: 3286323
• 负责人: JONATHAN W JARVIK
• 金额: $ 13.65万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-05 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3286323
• 关键词: Chlorophyta; affinity chromatography; alleles; cell cycle; cell motility; cell nucleus; centrosome; cytogenetics; electron microscopy; gel electrophoresis; gene duplication; gene expression; gene mutation; immunodiffusion; immunofluorescence technique; kinetosome; linkage mapping; microscopy; temperature sensitive mutant

As the Chlamydomonas cell grows its basal bodies follow a cycle of duplication, movement and segregation which is closely coupled to the cell cycle. A genetic analysis of this basal body cycle is proposed, with the emphasis on the analysis of mutants in which segregation is aberrant. Because the Chlamydomonas basal bodies serve to nucleate flagella in interphase, such mutants have a distinctive vfl (variable flagella number) phenotype by which they can readily be recognized. Light and electron microscopy will be used to describe the mutants cellular phenotypes in detail. The structural basis of basal body segregation is of particular interest to us, and so special attention will be given to the connectors which link basal bodies to the nucleus and to one another. Their behavior will be followed throughout the basal body cycle, both in wild type and in vfl mutants. The Chlamydomonas basal body cycle is closely homologous to the centriole cycle as it exists in higher eucaryotes. In spite of much intense investigation, the centriole's cellular function remains mysterious. The proposed investigation provides a unique opportunity to explore the function of this organelle whose essence has eluded biologists for over a century.

当衣藻细胞生长时，其基体遵循以下循环： 复制、运动和分离与细胞密切相关 循环。 提出了对该基础身体周期的遗传分析，其中 强调对分离异常的突变体的分析。 因为衣藻基体的作用是使鞭毛成核 间期，此类突变体具有独特的 vfl（可变鞭毛数量） 可以很容易地识别它们的表型。 光与电子 显微镜将用于描述突变体细胞表型 细节。 基底体分离的结构基础特别令人感兴趣 我们，因此将特别注意链接的连接器 基体与细胞核以及基体之间的关系。 他们的行为将会是 在野生型和 vfl 中的整个基础体周期中都遵循 突变体。 衣藻基础体循环与中心粒密切同源 高等真核生物中存在的循环。 尽管非常激烈 研究表明，中心粒的细胞功能仍然是个谜。 这 拟议的调查提供了一个独特的机会来探索 这个细胞器的功能，其本质一直困扰着生物学家超过一个多世纪 世纪。