IDENTIFICATION OF HUMAN HISTONE GENE REGULATORY SEQUENCE

人类组蛋白基因调控序列的鉴定

基本信息

批准号：
3280538
负责人：
Gary S. Stein
金额：
$ 14.09万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
起止时间：
1983-04-01 至 1986-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3280538
关键词：
cell cycle cell growth regulation developmental genetics gene expression gene mutation genetic manipulation genetic mapping genetic regulation genetic transcription messenger RNA nucleic acid sequence regulatory gene

项目摘要

IDENTIFICATION OF HUMAN HISTONE GENE REGULATORY SEQUENCES: The overall objective of our research program is to understand the structure, organization, and regulation of human histone genes. Histones are of key importance in determining structural and transcriptional properties of the genome. Additionally, histones and particularly histone synthesis appear to be intimately involved with genome replication. We have recently isolated and characterized a series of Lambda Charon 4A recombinant phage which contain human histone genes. In the studies described here, we propose to identify and characterize regulatory sequences associated with several actively transcribed human histone genes. Messenger RNA coding regions and the flanking sequences will then be identified by introduction of deletions and site-directed mutations, with assessment of transcription both in vivo and in vitro. To further define the functionality of DNA sequences involved in regulating histone gene transcription, we will exchange "regulatory" and "structural" sequences of human histone genes with those of another gene such as globin. Because at least a subset of the human histone genes are under cell cycle control, it should be possible to determine whether histone regulatory sequences can control expression of other genes in a cell cycle stage-specific manner.

人类组蛋白基因调节序列的识别：总体我们研究计划的目的是了解结构，组织和人类组蛋白基因的调节。组蛋白是关键的确定结构和转录特性的重要性基因组。此外，组蛋白，特别是组蛋白的合成出现与基因组复制密切相关。我们最近有孤立并表征了一系列Lambda Charon 4A重组噬菌体包含人类组蛋白基因。在这里描述的研究中，我们建议识别和表征与一些积极转录的人类组蛋白基因。 Messenger RNA编码然后将通过引言确定区域和侧翼序列删除和定位突变，并评估转录体内和体外。进一步定义DNA的功能涉及调节组蛋白基因转录的序列，我们将交换人类组蛋白基因的“调节”和“结构”序列与另一个基因（例如Globin）的那些。因为至少一个子集人组蛋白基因处于细胞周期控制之下，应该有可能确定组蛋白调节序列是否可以控制其他基因以细胞周期阶段特异性方式。