Multimodal coherent Raman and two-photon fluorescence imaging as a discovery tool in bioscience

多模态相干拉曼和双光子荧光成像作为生物科学的发现工具

基本信息

批准号：
BB/T017759/1
负责人：
Valerie Brunton
金额：
$ 38.62万
依托单位：
University of Edinburgh
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2020
资助国家：
英国
起止时间：
2020 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FT017759%2F1
关键词：
Multimodal coherent Raman two photon

项目摘要

Bioimaging is a fundamental technology underpinning bioscience and has been instrumental in unravelling the complexities of biological systems that control tissue development, differentiation and that drive disease processes. Our approach has been to combine label-free coherent Raman imaging modalities, which generate image contrast using the Raman active vibrational frequency of a given chemical, with two-photon fluorescence imaging modalities to provide insight into both subcellular and tissue organisation. Recent advances in biorthogonal chemistry which allows Raman tagging and subsequent visualisation of small drug molecules, drug delivery vehicles and cellular constituents has extended the benefit of this technology to a much wider scientific base.Several advances in instrument design have been made since we set up our current multimodal imaging platform in 2011 which means that we can significantly enhance and extend the imaging capabilities by increasing the stability, sensitivity, accuracy and speed of our measurements, while also enabling imaging of far-red fluorophores which are becoming increasingly popular in the design of in vivo probes due to the enhanced penetration of higher wavelengths into tissue. The new set-up will provide a unique platform to carry out intravital imaging in live animals, including mice and zebrafish, providing unrivalled insight into spatial and temporal control of fundamental biological processes. In addition, access to the expertise and knowledge of the consortium of researchers that have put together this proposal (who have an excellent track record of publications and securing financial support including from the BBSRC) will drive success and ensure maximal impact of the research undertaken. This will support scientific advances and enable technological innovation that will impact all areas of the biological sciences.The aim of this proposal is to provide a state-of-the-art multimodal imaging platform available to scientific communities across the UoE, other academic institutions throughout the UK, and our partners in industry. The proposal brings together a multidisciplinary consortium of researchers (chemists, biologists, engineers) with expertise in bioimaging with a broad range of diverse research goals. The new platform will therefore underpin scientific research in several key strategic BBSRC priorities for the next decade: combatting microbial resistance; 3Rs in research using animals; sustainably enhancing agricultural production; systems approaches to the biosciences; technology development for the biosciences; synthetic biology.

生物成像是支撑生物科学的基础技术，有助于揭示控制组织发育、分化和驱动疾病过程的生物系统的复杂性。我们的方法是将无标记相干拉曼成像模式（使用给定化学物质的拉曼主动振动频率生成图像对比度）与双光子荧光成像模式相结合，以提供对亚细胞和组织组织的洞察。双正交化学的最新进展允许小药物分子、药物输送载体和细胞成分的拉曼标记和随后的可视化，将这项技术的优势扩展到更广泛的科学基础。自从我们建立我们的实验室以来，仪器设计已经取得了一些进展。 2011 年我们推出了当前的多模态成像平台，这意味着我们可以通过提高测量的稳定性、灵敏度、准确性和速度来显着增强和扩展成像能力，同时还可以对远红荧光团进行成像，远红荧光团在设计中越来越受欢迎。由于较高波长对组织的穿透力增强，因此可用于体内探针。新装置将提供一个独特的平台，对小鼠和斑马鱼等活体动物进行活体成像，为基本生物过程的空间和时间控制提供无与伦比的见解。此外，获得撰写该提案的研究人员联盟的专业知识和知识（他们拥有出色的出版物记录并获得包括来自 BBSRC 的财政支持）将推动成功并确保所进行的研究产生最大影响。这将支持科学进步并实现影响生物科学所有领域的技术创新。该提案的目的是为整个英格兰大学的科学界和整个大学的其他学术机构提供最先进的多模态成像平台英国以及我们的工业合作伙伴。该提案汇集了一个由具有生物成像专业知识和广泛不同研究目标的多学科研究人员（化学家、生物学家、工程师）组成的联盟。因此，新平台将支持未来十年 BBSRC 几个关键战略优先事项的科学研究：对抗微生物耐药性；使用动物进行研究的 3R；可持续地提高农业生产；生物科学的系统方法；生物科学技术开发；合成生物学。