REGULATION OF EXOGENOUSLY INDUCED OPERON

外源诱导操纵子的调控

• 批准号: 3280191
• 负责人: JEN SHIANG HONG
• 金额: $ 18.26万
• 依托单位: BOSTON BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-04-01 至 1990-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3280191
• 关键词: DNA binding protein; Escherichia coli; Salmonella typhimurium; bacterial genetics; binding proteins; biological transport; crosslink; gene expression; genetic manipulation; genetic transcription; genetic translation; goats; immunochemistry; membrane proteins; molecular cloning; mutant; nucleic acid probes; nucleic acid sequence; operon; phosphoglycerate; radiotracer; regulatory gene; site directed mutagenesis

The genetic expression of the phosphoglycerate transport system of S. typhimurium is induced by external inducers but not by internal inducers. The long-term objectives of the proposed research are to gentically and biochemically characterize the regulatory machinery involved in gene expression of this transport system and to understand the biochemical and molecular mechanism underlying this novel gene regulation. During the current grant period we dissected the genetic regulatory machinery, determined the DNA sequence, identified gene products and determined the cellular locations of these products. The specific aims of the present proposal are: to isolate, purify and characterize the pgt regulatory proteins; to study the regulatory mechanism in in vitro transcription-translation coupled system in order to determine the functional role of each regulatory proteins with respect to inducer binding, DNA binding and signal transmission; to analyze pgt transcripts and determine pgtP transcription initiation start and DNA binding regions, in order to distinguish two models of regulation and to define the control regions; to isolate constitutive regulatory mutants by genetic selection and site-directed mutagenesis and to characterize the mutant proteins; to study processing of regulatoryp proteins and determine the cleavage sites; and to perform chemical cross-linking experiments to investigate the interaction of the regulatory proteins.

S.磷酸甘油酸转运系统的遗传表达 鼠伤寒是由外部诱导剂诱导的，而不是由内部诱导剂诱导的。 拟议的研究的长期目标是在绅士上和 生化表征了涉及基因的调节机制 该运输系统的表达并了解生化和 这种新基因调控的基础的分子机制。 在 当前的赠款期我们剖析了遗传调节机制， 确定了DNA序列，鉴定了基因产物并确定了 这些产品的细胞位置。 当前的具体目的 建议是：隔离，纯化和表征PGT调节 蛋白质；研究体外调节机制 转录翻译耦合系统，以确定 每个调节蛋白相对于诱导剂的功能作用 结合，DNA结合和信号传递；分析PGT成绩单 并确定PGTP转录起始开始和DNA结合区域， 为了区分两种调节模型并定义控制 地区；通过遗传选择分离本构调节突变体 和定向诱变并表征突变蛋白；到 调节性蛋白质的研究加工并确定切割位点； 并进行化学交联实验以研究 调节蛋白的相互作用。

项目成果

Identification of the products and nucleotide sequences of two regulatory genes involved in the exogenous induction of phosphoglycerate transport in Salmonella typhimurium.

鉴定参与鼠伤寒沙门氏菌磷酸甘油酸转运外源诱导的两个调控基因的产物和核苷酸序列。

• DOI: 10.1128/jb.170.9.4299-4303.1988
• 发表时间: 1988
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Yang,YL;Goldrick,D;Hong,JS
• 通讯作者: Hong,JS

Identification and nucleotide sequence of the activator gene of the externally induced phosphoglycerate transport system of Salmonella typhimurium.

外部诱导的鼠伤寒沙门氏菌磷酸甘油酸转运系统激活基因的鉴定和核苷酸序列。

• DOI: 10.1016/0378-1119(86)90131-9
• 发表时间: 1986
• 期刊: Gene
• 影响因子: 3.5
• 作者: Yu,GQ;Hong,JS
• 通讯作者: Hong,JS