FUTURE OAK: Characterising and engineering the oak microbiome to future-proof an arboreal icon

未来橡树：表征和改造橡树微生物组，打造面向未来的树栖标志

• 批准号: BB/T01069X/1
• 负责人: James McDonald
• 金额: $ 172.73万
• 依托单位: Bangor University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FT01069X%2F1
• 关键词: FUTURE; OAK; Characterising; engineering; oak

Tree declines caused by climate perturbation, anthropogenic stressors and disease outbreaks have resulted in recent forest mega-disturbances and are a major global concern. Until recently, progress has been hampered by a lack of high-throughput analytical approaches for systems-based analysis of the multidimensional factors that drive declines. Our previous research on Acute Oak Decline, a complex decline disease of native British oak, has highlighted how microbiome shifts to a 'pathobiome', are associated with tree disease. This work challenges the one pathogen = disease paradigm, leading to new hypotheses on the role of microbiomes in health and disease, and highlighting the need to revise classical disease paradigms to include the microbiome. The microbiome is a major determinant of plant health, and we hypothesise that the oak microbiome can be manipulated to improve host fitness and disease suppression. Here, we propose a landscape-scale analysis of the oak microbiome and metabolome in health and disease, to inform the design and validation of engineered microbiomes for disease suppression. Microbiome engineering is an emerging research frontier with many novel applications. The project will address new hypotheses regarding how complex multidimensional interactions between the tree host and its microbiota, encountered pathogens, and the environment, influence host fitness and disease susceptibility. The research will initiate a new frontier in forest microbiome engineering and precision medicine to future-proof iconic tree species and the ecosystem services they provide.

气候扰动、人为压力源和疾病爆发造成的树木减少导致了最近的森林特大扰动，成为全球关注的重大问题。直到最近，由于缺乏对导致衰退的多维因素进行系统分析的高通量分析方法，进展一直受到阻碍。我们之前对急性橡树衰退（一种英国本土橡树的复杂衰退疾病）的研究强调了微生物组如何转变为“病理组”，与树木疾病相关。这项工作挑战了单一病原体=疾病的范式，引发了关于微生物组在健康和疾病中的作用的新假设，并强调需要修改经典疾病范式以将微生物组包括在内。微生物组是植物健康的主要决定因素，我们假设可以操纵橡树微生物组来改善宿主的适应性和抑制疾病。在这里，我们提出对健康和疾病中的橡树微生物组和代谢组进行景观尺度分析，为用于抑制疾病的工程微生物组的设计和验证提供信息。微生物组工程是一个新兴的研究前沿，具有许多新颖的应用。该项目将提出新的假设，涉及树木宿主及其微生物群、遇到的病原体和环境之间复杂的多维相互作用如何影响宿主的适应性和疾病易感性。该研究将开创森林微生物组工程和精准医学的新前沿，以适应未来的标志性树种及其提供的生态系统服务。

项目成果

Chemical cues from beetle larvae trigger proliferation and virulence of a plant pathogen

甲虫幼虫的化学信号触发植物病原体的增殖和毒力

• DOI: http://dx.10.1101/2023.11.21.568124
• 发表时间: 2023
• 作者: Cambon M

"How to recognise a healthy forest: perspectives from private forest managers in Britain."

“如何认识健康的森林：英国私人森林管理者的观点。”

• 期刊: Forest Policy and Economics
• 影响因子: 4
• 作者: Dandy N

Spectral binning as an approach to post-acquisition processing of high resolution FIE-MS metabolome fingerprinting data

光谱分箱作为高分辨率 FIE-MS 代谢组指纹数据采集后处理的方法

• DOI: http://dx.10.1007/s11306-022-01923-6
• 发表时间: 2022
• 期刊: Metabolomics
• 影响因子: 3.6
• 作者: Finch J