EXPRESSION OF DROSOPHILA A-TUBULIN GENES

果蝇 A-微管蛋白基因的表达

• 批准号: 3279161
• 负责人: PIETER C WENSINK
• 金额: $ 12.96万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3279161
• 关键词: autoradiography; developmental genetics; endonuclease; gene expression; gene mutation; genetic manipulation; genetic mapping; genetic transcription; messenger RNA; nucleic acid sequence; structural genes

This project will investigate how Drosophila controls the expression of its Alpha-tubulin genes. The four genes of this small multigene family offer a good opportunity to investigate the control of tissue- and time-specific transcription and to investigate mRNA characteristics that determine mRNA stability and location. The issues to be dealt with in this investigation are: 1) the location and complexity of DNA sequences that cause one family member's transcription pattern to deiifer from that of another member; and 2) whether or not there are sequences in the mRNAs from different family members that cause them to differ in fate, that is to differ in intercellular transport, in cellular location and in stability. In pursing the first issue, regions and sub-regions will be exchanged between the Alpha-tubulin genes and the resulting genes used to transform Drosophila. The pattern of expression of these altered genes will indicate which sequences are necesssary for a particular tissue- or time- specific pattern of transcription. In pursuing the second issue, portions of the mRNA encoding regions of Alpha-tubulin genes will be excahanged with portions of other genes and then a transformation assay will be used to test the effect of 3 feet and 5 feet untranslated regions, introns and translated regions on the intercellular transport, the location and the stability of Alpha-tubulin mRNAs. The initial subjects for these exchanges will be an Alpha-tubulin gene which codes for a maternal mRNA, one which codes for an mRNA that first appears between egg laying and cellular blastoderm formation and one which appears to be transcribed at all times in all tissues.

该项目将研究果蝇如何控制其表达 α-微管蛋白基因。 这个小型多基因家族的四个基因提供了 研究组织和时间特异性控制的好机会 转录并研究决定 mRNA 的 mRNA 特征 稳定性和位置。 三、本次调查需要解决的问题 是：1）导致一个家庭的DNA序列的位置和复杂性 成员的转录模式与另一个成员的转录模式不同；和 2) 不同家族的mRNA中是否存在序列 导致他们命运不同的成员，即不同的人 细胞间运输、细胞定位和稳定性。 在追求第一期时，将交换区域和次区域 α-微管蛋白基因和用于转化的所得基因之间 果蝇。 这些改变的基因的表达模式将表明 哪些序列对于特定组织或时间特定是必需的 转录模式。 在讨论第二个问题时，部分 α-微管蛋白基因的 mRNA 编码区域将被交换 其他基因的部分，然后使用转化测定 测试 3 英尺和 5 英尺非翻译区域、内含子和 细胞间运输的翻译区域、位置和 α-微管蛋白 mRNA 的稳定性。 这些交流的最初主题 将是一个α-微管蛋白基因，它编码母体mRNA，其中 编码首先出现在产卵和细胞之间的 mRNA 胚盘的形成和似乎一直在转录的胚盘形成 在所有组织中。