THE COMPLEXITY OF CHROMOSOMAL STRUCTURE AND ASSEMBLY

染色体结构和组装的复杂性

• 批准号: 3273672
• 负责人: HAROLD M WEINTRAUB
• 金额: $ 15.23万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-05-01 至 1989-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3273672
• 关键词: Adenoviridae; chromatin; developmental genetics; early embryonic stage; electron microscopy; erythroid stem cell; erythropoiesis; gene expression; genetic manipulation; genetic mapping; genetic recombination; genetic transcription; microinjections; mutant; myogenesis; nonmammalian vertebrate embryology; radiotracer; tissue /cell culture; tritium

Genes that might control the determination of a specific cell lineage and genes that might regulate the progression of cells through that lineage have not been identified. We propose a series of DNA and RNA microinjection protocols based largely on recombinant DNA technology to identify genes that "regulate" the developmental transitions occuring during the later stages of erythropoiesis and granulopoiesis and the early stages of erythropoiesis. Since we believe that the action of these types of genes is both reflected and recorded in chromatin structure, a major effort will be devoted to the study of DNase I and S1 hypersensitive (H.S.) sites; and the role, if any, of H1 in gene expression. Specific experiments are proposed to study in situ the patterns of chromatin assembly and segregation. Continuing efforts to develop methods to study chicken lampbrush chromosomes should enable rapid gene localization and localization of specific proteins. These chromosomes may also be useful "reagents" for morphological visualization of nuclear structures previously seen only by biochemical methods.

可能控制特定细胞谱系确定的基因和 可能调节细胞通过该谱系进展的基因 尚未确定。 我们提出了一系列DNA和RNA 显微注射方案主要基于重组DNA技术 确定“调节”发生发展过渡的基因 在红细胞生成和颗粒状的后期阶段以及早期 红细胞生成的阶段。 因为我们相信这些类型的行动 基因的基因反映和记录在染色质结构中，这是一个主要的 努力将致力于研究DNase I和S1过敏（H.S.） 站点； H1在基因表达中的作用（如果有的话）。 具体的 提出了实验以原位研究染色质的模式 组装和隔离。 不断努力开发学习方法 鸡肉兰蒙染色体应实现快速基因定位和 特定蛋白质的定位。 这些染色体也可能有用 以前用于核结构形态可视化的“试剂” 仅通过生化方法看。