Understanding how RNA is tethered to chromatin

了解 RNA 如何与染色质结合

• 批准号: BB/V00722X/1
• 负责人: Stuart Wilson
• 金额: $ 60.7万
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FV00722X%2F1
• 关键词: Understanding; how; RNA; tethered; chromatin

This project addresses where RNA is localised in the cell. Location is very important as any estate agent will tell you. The mammalian cell produces a wide range of RNA molecules with diverse functions. Some well known RNAs, such as messenger RNA, are produced in the nucleus and then exported to the cytoplasm where they encode for proteins. Many other types of RNA largely reside in the nucleus and some of them are tethered to the DNA where they regulate the production of RNA and play a role in the overall structural organisation of the nucleus. We have discovered that the HNRNPU protein plays a widespread role in tethering RNAs to DNA in the nucleus and additionally is probably involved in tethering different RNAs to each other. In this project we will characterise the nuclear RNA:DNA and RNA:RNA tethering mechanisms controlled by HNRNPU and determine the impact these processes have on mammalian gene expression.

该项目解决了RNA位于细胞中的位置。任何房地产经纪人都会告诉您的位置非常重要。哺乳动物细胞产生具有不同功能的各种RNA分子。一些众所周知的RNA（例如Messenger RNA）在细胞核中产生，然后导出到细胞质中，并在其中编码蛋白质。许多其他类型的RNA很大程度上驻留在细胞核中，其中一些被束缚在DNA上，在DNA中它们调节RNA的产生，并在核的整体结构组织中发挥作用。我们已经发现，HNRNPU蛋白在将RNA绑定到核中的DNA中起着广泛的作用，并且可能参与将不同的RNA彼此绑定。在这个项目中，我们将表征核RNA：DNA和RNA：由HNRNPU控制的RNA绑扎机制，并确定这些过程对哺乳动物基因表达的影响。