LIPID METABOLISM AND MEMBRANE STRUCTURE AND FUNCTION

脂质代谢与膜结构和功能

• 批准号: 3270817
• 负责人: MICHAEL GLASER
• 金额: $ 17.88万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-01-01 至 1990-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3270817
• 关键词: Escherichia coli; Rous sarcoma virus; adenylate cyclase; adenylate kinase; cell growth regulation; circular dichroism; cytochrome b5 reductase; electron spin resonance spectroscopy; electron transport; endoplasmic reticulum; fluorescence spectrometry; fluorimetry; high performance liquid chromatography; lipid metabolism; membrane activity; membrane lipids; membrane reconstitution /synthesis; membrane structure; mitochondria; molecular polarity; mutant; nuclear magnetic resonance spectroscopy; phospholipids; radiotracer; temperature sensitive mutant; tissue /cell culture; virus envelope

The objective of this research is to obtain an understanding of the role of lipids, especially the phospholipid polar head groups, in membrane structure and function. The main approach to be used in this research will be to alter the lipid composition with respect to the fatty acids, the polar head groups and the sterol content of cells in tissue culture. Subcellular membrane fractions, including the plasma membrane, endoplasmic reticulum, and mitochondria will be isolated from the cells with different lipid compositions. The changes in the lipid composition will be correlated with changes in the physical and biochemical properties of the membranes. The structure and fluidity of the membranes and reconstituted phospholipid vesicles containing membrane proteins will be studied by fluorescence polarization, energy transfer methods, and phase fluorometry. The properties of D-Beta-hydroxybutyrate dehydrogenase, cytochrome b5 and cytochrome b5 reductase will be examined in order to determine how lipids interact with them and affect their activity. Another aspect of the research will involve the properties of vesicular stomatitis virus and Rous sarcoma virus derived from cells with different lipid compositions in order to understand how these viruses acquire their unique lipid compositions. Along the same lines, the properties of lipoproteins secreted by primary rat hepatocyte cultures will be examined to determine if the lipoproteins selectively incorporate phospholipids. These studies should provide unique information on the contribution of lipids to the activity of membranous enzymes and to the organization and structure of animal cell membranes and viruses.

这项研究的目的是了解 脂质，尤其是磷脂极性头基在膜上 结构和功能。 这项研究中要使用的主要方法将 要改变脂质成分相对于脂肪酸， 极性头部组和组织培养中细胞的固醇含量。 亚细胞膜分数，包括质膜，内质。 网状和线粒体将从不同的细胞中分离出来 脂质成分。 脂质成分的变化将是 与物理和生化特性的变化相关 膜。 膜的结构和流动性和重构 将研究含有膜蛋白的磷脂囊泡 荧光偏振，能量转移方法和相荧光法。 D-beta-羟基丁酸脱氢酶，细胞色素B5和 将检查细胞色素B5还原酶，以确定脂质 与他们互动并影响他们的活动。 的另一个方面 研究将涉及囊泡口腔炎病毒和爆发的特性 从具有不同脂质组成的细胞衍生的肉瘤病毒按顺序 了解这些病毒如何获得其独特的脂质成分。 同样，主要的脂蛋白特性由原发性分泌 将检查大鼠肝细胞培养物以确定脂蛋白是否是 有选择地掺入磷脂。 这些研究应该提供独特的 有关脂质对膜活动的贡献的信息 酶以及动物细胞膜的组织和结构和结构 病毒。