CHROMOSOMAL FLOW CYTOMETRY--DNA AND CENTROMERES

染色体流式细胞术--DNA 和着丝粒

• 批准号: 3272761
• 负责人: JOE N LUCAS
• 金额: $ 16.76万
• 依托单位: UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-04-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3272761
• 关键词: DNA; centromere; chromosome disorders; clinical biomedical equipment; computer assisted diagnosis; computer simulation; cytogenetics; eukaryote; flow cytometry; fluorescence; histochemistry /cytochemistry; karyotype; light scattering; stainings

The long term objective of this project is to develop flow cytometric devices and procedures for rapid, quantitative classification of chromosomes according to shape and/or fluorescent banding pattern. These developments will contribute to cytogenetic studies by allowing quantitation of important cytogenetic endpoints such as: 1) polyploidy, trisomy, and monosomy, 2) chromosomal variants such as stain content polymorphisms, and 3) structural rearrangements such as fragments, deletions, dicentrics, and translocations. In this project, we propose to continue development of flow cytometric procedures for measurement of the distribution of fluorescent dye(s) along isolated mammalian chromosomes. Specifically we will: complete development of a slit-scan flow cytometer capable of measuring and classifying chromosomes according to shape at rates up to 1000/sec with spatial resolution approaching 2.5 Mum; develop fringe-scan flow cytometry to allow chromosome scanning and classification with spatial resolution approaching 0.3 Mum, and develop improved chromosome preparation and staining procedures to produce enlarged, fluorescently banded chromosomes. As these new chromosome scanning techniques are developed, we will use them for quantification of the frequency of randomly occurring aberrant chromosomes in mutagen treated cell populations and for classification of homogeneously chromosomes according to shape and/or banding pattern.

该项目的长期目标是发展流式细胞术 快速，定量分类的设备和程序 根据形状和/或荧光带模式的染色体。 这些 发展将通过允许 定量重要的细胞遗传学终点，例如：1）多倍体， 三体和单切，2）染色体变体，例如染色含量 多态性和3）结构重排，例如碎片， 删除，dicentrics和易位。 在这个项目中，我们建议继续开发流式细胞仪 测量荧光染料分布的程序 孤立的哺乳动物染色体。 具体我们将：完成 开发缝隙扫描流式细胞仪，能够测量和 根据形状以高达1000/sec的速率对染色体进行分类 接近2.5妈妈的空间分辨率；发展边缘扫描流式细胞仪 允许使用空间分辨率进行染色体扫描和分类 接近0.3妈妈，并产生改善的染色体制备和 染色程序可产生扩大的荧光带状染色体。 随着这些新的染色体扫描技术的开发，我们将使用它们 用于定量随机发生异常的频率 诱变治疗的细胞群体中的染色体和用于分类 根据形状和/或带状模式均质染色体。