STRUCTURE AND CONFORMATION OF SIDEROPHORES AND PEPTIDES

铁载体和肽的结构和构象

• 批准号: 3270704
• 负责人: DICK VAN DER HELM
• 金额: $ 15.05万
• 依托单位: UNIVERSITY OF OKLAHOMA
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-05-01 至 1990-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3270704
• 关键词: Candida albicans; Fungi; Fusarium; Mossbauer spectrometry; Neurospora; X ray crystallography; active transport; aluminum; antibiotics; bacteria; calcium; chemical bond; chromium; circular dichroism; cobalt; conformation; crystallization; cyclic peptides; hemoprotein structure; hydroxamate; iron metabolism; lactams; magnesium; metal complex; metalloproteins; molecular rearrangement; nuclear magnetic resonance spectroscopy; nucleotides; physical separation; radiotracer; stereoisomer

1) Isolation, structure determination by single crystal X-ray diffraction, and NMR of siderophores and ferrisiderophores from the iron-deficient media of fungi and bacteria pathogenic to man, insects and plants, as well as siderophores from mycorrhyzal and nitrogen fixing species. 2) Identification, isolation, purification, crystallization and structure determination (X-ray) of the ferrisiderophore receptor protein in Aspergillus ochraceous or the 81 K M.W. ferric enterobactin receptor protein in E. coli K 12. 3) Kinetics and mechanism of ferrisiderophores-uptake in microorganisms from which they are produced. 4) Molecular mechanics calculations to investigate the absolute configuration of siderophores and the flexibility of cyclic peptides. 5) Synthesis of several selected self-duplexing nucleotides and the structure determination by X-ray and NMR of these nucleotides and their complex with a double intercalating agent.

1）通过单晶X射线衍射的隔离，结构测定， 来自铁缺陷培养基的铁载体和铁骨载体的NMR 真菌和细菌对人，昆虫和植物的致病性 来自霉菌和氮固定物种的铁载体。 2）识别，隔离，纯化，结晶和结构 在铁丝体受体蛋白中测定（X射线） 曲霉菌或81 k M.W.摄影素受体 大肠杆菌K 12中的蛋白质。 3）微生物中的亚麻载体摄取的动力学和机制 它们是从中生产的。 4）分子力学计算以研究绝对 铁载体的构型和环状肽的灵活性。 5）合成几种选择的自叠加核苷酸和 这些核苷酸及其X射线和NMR的结构测定 与双重插入剂的复合物。