CHARACTERIZATION OF BASEMENT MEMBRANE GLYCOPROTEINS

基底膜糖蛋白的表征

• 批准号: 3273243
• 负责人: ALBERT E CHUNG
• 金额: $ 15.21万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-01-01 至 1988-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3273243
• 关键词: basement membrane; carbohydrate structure; complementary DNA; crosslink; cyclic AMP; embryo /fetus tissue /cell culture; embryo neoplasm; endoderm; gel electrophoresis; gel filtration chromatography; gene expression; germ cell neoplasms; hybrid cells; hybridomas; immunochemistry; messenger RNA; molecular cloning; monoclonal antibody; radiotracer; retinoate; tissue /cell culture

The long term objective of this proposal is to understand the organization of basement membranes and their role in normal and pathological states. Basement membranes are complex extracellular matrices that are involved in tissue organization, embryogenesis, cell adhesion, filtration and protection. There are alterations in basement membrane structures in many pathological states such as diabetes mellitus, cancer, bullous pemphigoid, asthma, glomeruluo-nephritis of varied origin and several of the autoimmune diseases. The specific objectives of this proposal are two fold: (a) basement membrane organization will be studied with a model matrix produced in cell culture by mouse endoderm cells and (b) the regulation of the genes for laminin and entactin, two basement membrane components will be explored. The techniques that will be employed include the construction of hybridoma cells to generate specific probes for examining basement membrane organization, peptide isolation and sequencing, isolation and characterization of DNA sequences that code for the genes of laminin and entactin, and induction of embryonal carcinoma F9 cells with retinoic acid and dibutyryl cyclic AMP to study the expression of laminin and entactin.

该提议的长期目标是了解组织 地下膜及其在正常和病理状态中的作用。 地下膜是复杂的细胞外矩阵 组织组织，胚胎发生，细胞粘附，过滤和 保护。 许多人的基底膜结构发生了变化 病理状态，例如糖尿病，癌症，大声刺激性， 哮喘，不同来源的肾小球肾炎和几种自身免疫性 疾病。 该提案的具体目标是两个折：（a） 将使用产生的模型矩阵研究地下膜组织 在小鼠内胚细胞和（b）基因的调节中，在细胞培养中 对于层粘连蛋白和Entactin，两个地下膜成分将是 探索。 将采用的技术包括建造 杂交瘤细胞生成特定探针检查地下膜 组织，肽隔离和测序，隔离和 DNA序列的表征，这些序列编码层粘连蛋白基因和 肠子素，并用视黄酸诱导胚胎癌F9细胞 和二丁酰基循环AMP研究层粘连蛋白和肠蛋白的表达。