Characterisation of a novel bacterial ribonucleoprotein complex analogous to eukaryotic processing (P) bodies in Escherichia coli

类似于大肠杆菌中的真核加工 (P) 体的新型细菌核糖核蛋白复合物的表征

• 批准号: BB/V000284/1
• 负责人: Sivaramesh Wigneshweraraj
• 金额: $ 72.17万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FV000284%2F1
• 关键词: Characterisation; novel; bacterial; ribonucleoprotein; complex

Bacteria need nutrients to grow. However, in the environment or in the human body, bacterial growth is often compromised by nutrient limitation. Hence, bacteria spend the majority of their time in a starved and thus non-growing state. The processes that allow bacteria to cope with nutrient starvation begin with the synthesis of RNA - the first step in the reaction that switches on genes. Therefore, it is important to understand how the RNA, once synthesized, is managed to allow bacterial cope with nutrient starvation. This will allow us design novel interventional strategies to combat disease causing bacteria. In this project, since nitrogen represents an essential element of most molecules in the bacterial cell, we will use nitrogen starvation as a model nutrient stress to study in detail, how the bacterium Escherichia coli manages RNA. In particular, we will study in detail a novel 'site of RNA storage' in the E. coli, which we discovered to play an important role in how E. coli copes with nitrogen starvation. We posit that this 'site of RNA storage' could be akin to a similar feature, called the P-body, which is often formed in stressed cells found in our bodies and that of other animals. In summary, the results of this project will advance our fundamental knowledge of how the bacterial cell functions and thereby provide us with the much-needed new information and inspiration to control disease causing bacteria.

细菌需要营养才能生长。但是，在环境或人体中，细菌生长通常受到养分限制的损害。因此，细菌将大部分时间都花在饿死的状态下。允许细菌应对营养饥饿的过程始于RNA的合成 - 转化基因的反应的第一步。因此，重要的是要了解一旦合成的RNA如何设法允许细菌与营养饥饿。这将使我们设计新颖的介入策略来打击引起细菌的疾病。在该项目中，由于氮代表了细菌细胞中大多数分子的重要元素，因此我们将使用氮饥饿作为模型的养分应激，以详细研究，细菌大肠杆菌如何管理RNA。特别是，我们将详细研究大肠杆菌中新型的“ RNA存储位置”，我们发现它在大肠杆菌如何应对氮饥饿中起着重要作用。我们认为，这种“ RNA存储的位点”可能类似于类似的特征，称为P体，该特征通常是在我们体内和其他动物的压力细胞中形成的。总而言之，该项目的结果将促进我们对细菌细胞功能的基本知识，从而为我们提供急需的新信息和灵感，以控制引起细菌的疾病。