CARBON-13 MAGNETIC RESONANCE - METHODS AND THEORY

碳 13 磁共振 - 方法和理论

基本信息

批准号：
3268004
负责人：
David Morris Grant
金额：
$ 13.86万
依托单位：
UNIVERSITY OF UTAH
依托单位国家：
美国
项目类别：
财政年份：
1978
资助国家：
美国
起止时间：
1978-06-01 至 1988-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3268004
关键词：
bacteria chemical models chemical structure deuterium isoprenoid macromolecule membrane nuclear magnetic resonance spectroscopy nucleic acid structure nucleosides peptides relaxation spectrometry transfer RNA

项目摘要

This proposal outlines the continued development of methodology and theory using carbon-13 magnetic resonance important in the study of biomedical problems. Theoretical work largely deals with diffusional motion of biomedical molecules and model systems required to verify the experimental methods. Spectroscopic developments include slowspinning director alignment of nematic solvents to simulate molecular orienting conditions observed in biologically ordered systems such as membranes. Spectroscopic research will use high magnetic fields to determine subtle conformational features associated with optical centers in isoprenoid molecules. Relaxation data on continuous chain alkanes, model polymers, and biomacromolecules is used to characterize molecular motions of the overall and segmental variety. Solid state NMR methods using both cross polarization (CP) and magic angle spinning (MAS) provide relatively high resolution spectra. Applications primarily to nitrogen-containing compounds, heterocycles, nucleosides, nucleic acid, etc., are planned. Subtle interactions which average in liquid samples can be frozen out in microcrystalline powders for study. Finally, work on site specific 2H/1H isotope ratios and their variations depending upon the compounds' chemical history will be pursued. The effect of various biosynthetic pathways will be explored.

该提议概述了方法论和理论的持续发展使用在生物医学研究中重要的碳13磁共振问题。理论工作主要涉及验证实验所需的生物医学分子和模型系统方法。光谱发展的发展包括慢速导演列溶剂的排列以模拟分子定向条件在生物学有序的系统（例如膜）中观察到。光谱学研究将使用高磁场来确定微妙的构象与异on-分子中的光学中心相关的特征。关于连续链烷烃，模型聚合物和生物大分子用于表征整体的分子运动和分段品种。使用两个交叉的固态NMR方法极化（CP）和魔术角旋转（MAS）提供相对较高的分辨率光谱。主要应用于含氮的应用计划了化合物，杂环，核苷，核酸等。可以将平均液体样品中平均水平的微妙相互作用冻结在用于研究的微晶粉末。最后，在特定于现场的2H/1H上工作同位素比及其变化取决于化合物的化学历史将被追求。各种生物合成途径的影响将被探索。