ISOLATION OF VISUAL PATHWAY MUTANTS IN THE FISH

鱼视觉通路突变体的分离

• 批准号: 3265719
• 负责人: WALTER GILBERT
• 金额: $ 39.97万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-01 至 1994-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3265719
• 关键词: alternatives to animals in research; aquatic organism; autosomal recessive trait; biological models; embryo /fetus; fish; fresh water environment; gene expression; genetic regulatory element; genome; model design /development; molecular cloning; molecular genetics; mutant; neuroanatomy; neurogenesis; neurophysiology; nontherapeutic iontophoresis; parthenogenesis; reporter genes; tissue /cell culture; transposon /insertion element; vision disorders; visual pathways

This project seeks to understand the development of the vertebrate visual system using genetic analysis. By producing mutations via insertional DNA techniques we will be able to identify genes responsible for normal visual development in a vertebrate. We will insert DNA into the germline of fish species that: (1) have a short generation time; (2) can be scored rapidly for recessive mutations by artificial parthenogenesis; (3) can be cloned; and (4) are easily scored for visual defects. Some of the insertions will cause changes in the normal development of the visual system. Some of these changes should provide basic insights into this complex process. We will use anatomy, physiology, and biochemistry to analyze these mutants. We will apply a host of modern molecular biological techniques to identify and characterize the genes, the function of which is known from their phenotypes.

该项目旨在了解脊椎动物视觉的发展 使用遗传分析的系统。通过插入 DNA 产生突变 技术我们将能够识别负责正常视觉的基因 脊椎动物的发育。 我们将把 DNA 插入到以下鱼类的种系中：(1) 具有 生成时间短； (2)可以快速对隐性突变进行评分 通过人工孤雌生殖； (3)可克隆；和（4）很容易 对视觉缺陷进行评分。一些插入会引起变化 视觉系统的正常发育。其中一些改变应该 提供对这一复杂过程的基本见解。 我们将使用解剖学、生理学和生物化学来分析这些 突变体。我们将应用一系列现代分子生物学技术 识别和表征基因，其功能已知 他们的表型。