Multimodal coherent Raman and two-photon fluorescence imaging as a discovery tool in bioscience

多模态相干拉曼和双光子荧光成像作为生物科学的发现工具

• 批准号: BB/T017759/1
• 负责人: Valerie Brunton
• 金额: $ 38.62万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FT017759%2F1
• 关键词: Multimodal; coherent; Raman; two; photon; Multimodal; coherent; Raman; two; photon

Bioimaging is a fundamental technology underpinning bioscience and has been instrumental in unravelling the complexities of biological systems that control tissue development, differentiation and that drive disease processes. Our approach has been to combine label-free coherent Raman imaging modalities, which generate image contrast using the Raman active vibrational frequency of a given chemical, with two-photon fluorescence imaging modalities to provide insight into both subcellular and tissue organisation. Recent advances in biorthogonal chemistry which allows Raman tagging and subsequent visualisation of small drug molecules, drug delivery vehicles and cellular constituents has extended the benefit of this technology to a much wider scientific base.Several advances in instrument design have been made since we set up our current multimodal imaging platform in 2011 which means that we can significantly enhance and extend the imaging capabilities by increasing the stability, sensitivity, accuracy and speed of our measurements, while also enabling imaging of far-red fluorophores which are becoming increasingly popular in the design of in vivo probes due to the enhanced penetration of higher wavelengths into tissue. The new set-up will provide a unique platform to carry out intravital imaging in live animals, including mice and zebrafish, providing unrivalled insight into spatial and temporal control of fundamental biological processes. In addition, access to the expertise and knowledge of the consortium of researchers that have put together this proposal (who have an excellent track record of publications and securing financial support including from the BBSRC) will drive success and ensure maximal impact of the research undertaken. This will support scientific advances and enable technological innovation that will impact all areas of the biological sciences.The aim of this proposal is to provide a state-of-the-art multimodal imaging platform available to scientific communities across the UoE, other academic institutions throughout the UK, and our partners in industry. The proposal brings together a multidisciplinary consortium of researchers (chemists, biologists, engineers) with expertise in bioimaging with a broad range of diverse research goals. The new platform will therefore underpin scientific research in several key strategic BBSRC priorities for the next decade: combatting microbial resistance; 3Rs in research using animals; sustainably enhancing agricultural production; systems approaches to the biosciences; technology development for the biosciences; synthetic biology.

生物成像是一种基础的生物科学基础技术，并且在揭示控制组织发育，分化和推动疾病过程的生物系统的复杂性方面发挥了作用。我们的方法是将无标签的相干拉曼成像方式结合在一起，该模式使用给定化学物质的拉曼主动振动频率与两光子荧光成像方式产生图像对比度，以提供对亚细胞和组织组织的见识。生物融合化学反应的最新进展，该化学允许小型药物分子，药物输送车辆和细胞成分的随后可视化和随后的可视化，这将这项技术的好处扩大到了更广泛的科学基础上。自从我们在2011年以来，我们就可以在2011年实现了迅速的稳定性，并且可以在2011年建立仪器设计的范围，从而扩大了稳定性，并扩展了稳定性，并扩展了稳定性，并扩展了稳定性，并且能够扩展能力，并扩展了稳定性，并扩大了能力，并且能够扩展能力，并扩大了成像的稳定性。在我们的测量中，由于较高的波长渗透到组织中，因此可以对远红色的荧光团进行成像，这些成像在体内探针的设计中变得越来越流行。新的设置将提供一个独特的平台，以在包括小鼠和斑马鱼在内的活动物中进行静脉成像，从而为基本生物学过程的空间和时间控制提供无与伦比的见解。此外，访问研究人员联盟的专业知识和知识，这些研究人员将这项提案汇总在一起（他们具有出色的出版物记录并获得包括BBSRC（BBSRC）（包括来自BBSRC）的财务支持）将带来成功并确保所进行的研究的最大影响。这将支持科学进步并实现影响生物科学所有领域的技术创新。该提案的目的是为UOE的科学社区，整个英国的其他学术机构以及我们的行业合作伙伴提供一个最先进的多模式成像平台。该提案汇集了一个跨学科研究人员（化学家，生物学家，工程师）的跨学科财团，并具有多种不同的研究目标方面的专业知识。因此，新平台将在未来十年的几个关键战略BBSRC优先级中进行科学研究：对抗微生物抗性； 3R在研究中使用动物；可持续增强农业生产；系统接近生物科学；生物科学的技术发展；合成生物学。