GENETIC CONTROL OF DEVELOPMENT

发育的遗传控制

• 批准号: 3269641
• 负责人: JAMES Alfred HOCH
• 金额: $ 36.74万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-05-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3269641
• 关键词: Bacillus subtilis; S adenosylmethionine; affinity chromatography; autoradiography; bacterial DNA; bacterial genetics; beta galactosidase; cell differentiation; developmental genetics; endonuclease; enzyme mechanism; gel electrophoresis; gene expression; gene induction /repression; genetic manipulation; genetic mapping; genetic regulation; genetic transcription; protein engineering; protein structure; radiotracer; scintillation counter; site directed mutagenesis; sporogenesis; subtilisins; temperature sensitive mutant

The initiation of sporulation in Bacillus subtilis in controlled by five genes, spo0A, spo0B, spo0E, spo0F, and spo0H. The mechanism by which the products of these genes are able to control the transcription of key genes required for the initiation of sporulation as well as the synthesis of enzymes produced during the onset of stationary phase is unknown. One view of the role of the spo0 gene products in the control of the initiation of sporulation is focused around the function of the spo0A protein coded by the spo0A locus. The results are consistent with the idea that the activity of the spo0A protein is modulated by the products of the spo0B, spo0E and spo0F loci. The modified spo0A protein is thought to be a transcription factor or at least to influence transcription required for expression of certain key genes in the sporulation process. The research proposed in this application has three basic primary aims: 1) A detailed study of the subtilisin promoter as a paradigm for the action of spo0 products in transcription. 2) A study of the control of the expression of and the function of spo0 gene products by determination of the interrelationships of these products with themselves and with other components of the cell. 3) A detailed study of the function and structure of the spo0A protein will be undertaken through the use of site-directed mutagenesis techniques in order to gain a complete understanding of the role of this protein in the transcriptional activation of sporulation.

在受控的 五个基因，SPO0A，SPO0B，SPO0E，SPO0F和SPO0H。 这 这些基因的产物能够通过的机制 控制开始所需的关键基因的转录 孢子以及在期间产生的酶的合成 固定相的开始尚不清楚。 一个角色的观点 Spo0基因产物控制着 孢子形成围绕SPO0A蛋白的功能 由SPO0A基因座编码。 结果与 SPO0A蛋白的活性是由 SPO0B，SPO0E和SPO0F基因座的产品。 修改后的SPO0A 蛋白质被认为是转录因子，至少是 表达某些键所需的影响转录 基因在孢子形成过程中。 这项研究提出了 应用具有三个基本主要目的：1）详细研究 枯草素启动子作为SPO0作用的范式 转录产品。 2）对控制的研究 Spo0基因产物的表达和功能 确定这些产品的相互关系 本身以及细胞的其他组件。 3）详细的 SPO0A蛋白的功能和结构的研究将是 通过使用定向诱变而进行 为了完全了解角色的技术 该蛋白在孢子型的转录激活中的。