NEURAL CONTROL OF LACRIMAL GLANDS

泪腺的神经控制

基本信息

批准号：
3263866
负责人：
BENJAMIN WALCOTT
金额：
$ 14万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
起止时间：
1983-04-01 至 1989-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3263866
关键词：
B lymphocyte antibody formation electron microscopy histochemistry /cytochemistry immunochemistry immunoglobulin A lacrimal apparatus microscopy neural information processing norepinephrine secretion tear vasoactive intestinal peptide

项目摘要

The Harderian gland is a major lacrimal gland found in all animals with a nictitating membrane. The gland contains two major cell populations, a tubular secretory epithelium which secretes a serous fluid and lymphoid tissue consisting primarily of B-cells which secrete IgA. The gland is densely innervated and our preliminary data show that noradrenalin (NA) containing fibers are present as well as substance P-like, VIP-like and eukephalin-like immunoreactivity. Further these anatomical studies show that NA fibers are morphologically closer to the B-cells ihan to other cell types. Since there is some evidence for neuronal modulation of antibody production in spleen we will test this hypothesis in Harderian gland where antibody production in the tears can be measured. We will use immunocytochemical techniques at the light and electron microscope levels to determine the details of the innervation pattern. We will examine the pattern in chicks at different ages as the full complement of B-cells is not found until about 1 month before hatching. We will collect tears of normal and stimulated birds to see if antibody production is regulated as is secretion of the serous fluid.

硬腺是在所有动物中发现的主要富富腺刺激膜。腺体包含两个主要细胞群，一个管状分泌上皮，分泌浆液和淋巴样主要由分泌IgA的B细胞组成的组织。腺体是密集的支配，我们的初步数据表明甲肾上腺素（NA）存在包含纤维，以及类似p的物质，贵宾状和厄克塞林样免疫反应性。此外，这些解剖学研究表明 Na纤维在形态上更靠近B细胞IHAN靠近其他细胞类型。由于有一些证据表明抗体的神经元调节在脾脏中生产我们将在Harderian腺上检验该假设可以测量眼泪中的抗体产生。我们将使用光和电子显微镜水平的免疫细胞化学技术确定神经模式的细节。我们将检查由于B细胞的完整补充是不同年龄的小鸡的模式直到孵化前约1个月才发现。我们将收集眼泪正常和受刺激的鸟类，以查看是否调节抗体的产生是浆液的分泌。