EYE INFECTION OF CYTOMEGALOVIRUS

巨细胞病毒眼部感染

• 批准号: 3265147
• 负责人: JANG O OH
• 金额: $ 22.93万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-05-01 至 1994-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3265147
• 关键词: Betaherpesvirinae; T lymphocyte; cellular immunity; eye infections; genome; helper T lymphocyte; histopathology; immunosuppression; laboratory mouse; latent virus infection; macrophage; monoclonal antibody; natural killer cells; nucleic acid hybridization; nucleic acid probes; suppressor T lymphocyte; tissue /cell culture

Many ocular diseases of cytomegalovirus (CMV) in humans are opportunistic infections of the eye and occur predominantly in immunosuppressed individuals. As the number of immunosuppressed patients increases in recent years due largely to acquired immunodeficiency syndrome (AIDS), CMV has become the causal agent for an important eye disease. Yet, the pathogenesis of CMV eye infection is poorly understood at present. In view of ten serious nature of CMV eye infection and even increasing incidence of CMV eye infection among AIDS patients, a critical assessment of the role of CMV in human eye infection as well as a comprehensive study of the nature of the disease are urgently needed. Our preliminary studies on murine CMV (MCMV) eye infection clearly showed that as in humans, the intact host cellular immunity is an important factor for the protection of the mouse from the infection and recurrence of MCMV. Therefore, as our initial efforts to evaluate the effect of host cellular immunity on the pathogenesis of CMV eye infection, the following series of experiments is proposed: 1) Roles of various cellular immune components of the mouse, namely helper T cells (L3T4+ cells), suppressor T cells (Lyt-2+ cells), natural killer cells and macrophages in the induction of viremia and primary eye infection during systemic infection of MCMV will be studied. 2) The establishment of latency of MCMV in the eye and macrophages following primary infection will be examined, and the location of the virus genomes in the eye tissues will be determined by means of co-culture method and DNA-DNA hybridization techniques with MCMV-DNA probes. 3) Cellular immune components which may be responsible for in vivo reactivation of latent MCMV in the eye will be identified by evaluating the effect of selective depletion of each immune component on the reactivation of the latent virus. The proposed study will provide us hitherto unavailable information on CMV eye infection which will shed some light on our understanding of pathogenesis of the disease and also will assist us in the search for effective means for the prevention and treatment of CMV infections in humans.

人类中巨细胞病毒（CMV）的许多眼部疾病是 眼睛的机会感染，主要发生 免疫抑制的人。 作为免疫抑制的数量 近年来患者的增加很大程度上是由于获得的 免疫缺陷综合征（AIDS），CMV已成为因果剂 对于重要的眼科疾病。 然而，CMV眼的发病机理 目前，感染尚不清楚。 鉴于十个严重 CMV眼感染的性质，甚至CMV的发生率增加 艾滋病患者的眼部感染，对 CMV在人眼感染中的作用以及全面的研究 迫切需要疾病的性质。 我们对鼠CMV（MCMV）眼部感染的初步研究 表明与人类一样，完整的宿主细胞免疫是 保护小鼠免受感染的重要因素 和MCMV的复发。 因此，作为我们最初的努力 评估宿主细胞免疫对发病机理的影响 CMV眼感染，以下一系列实验是 建议的： 1）小鼠各种细胞免疫成分的作用，即 助手T细胞（L3T4+细胞），抑制T细胞（Lyt-2+细胞）， 天然杀手细胞和巨噬细胞诱导病毒血症 MCMV全身感染期间的原发性眼感染将 被研究。 2）在眼睛和巨噬细胞中建立MCMV的潜伏期 将检查原发性感染之后，并 眼组织中的病毒基因组将通过均值确定 共培养方法和DNA-DNA杂交技术与 MCMV-DNA探针。 3）可能负责体内的细胞免疫成分 眼睛中潜在MCMV的重新激活将通过 评估每种免疫的选择性耗竭的效果 潜在病毒重新激活的成分。 拟议的研究将为我们提供迄今无法获得的信息 在CMV眼感染上，这将在我们的 了解该疾病的发病机理，也将有助于 我们为预防寻求有效手段和 治疗人类CMV感染。