Ultra High-Throughput Sequencing for Norwich Research Park and the UK National Capability in Genomics

诺维奇研究园和英国国家基因组学能力的超高通量测序

• 批准号: BB/R014329/1
• 负责人: Neil Hall
• 金额: $ 97.84万
• 依托单位: Earlham Institute
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR014329%2F1
• 关键词: Ultra; Throughput; Sequencing; Norwich; Research

The ability to rapidly and cheaply sequence the genomes of living organisms has revolutionised biological and biomedical research. In the last 10 years the technology to enable this type of research as improved massively, as has our ability to understand the data. Genome sequencing has changed from an activity that required a bespoke factory scale facility to something that requires a single instrument which can be run in university labs across the UK and now even in hand-held devices. However, as the process has become quicker and cheaper, we can now design much larger and more powerful studies and there is still a need for bespoke facilities that can handle the larger study designs that are now possible. Here we propose to purchase the most recent iteration of short-read sequencing technology, The NovaSeq 6000 and install it at the Earlham Institute, which hosts the BBSRC National Capability in Genomics and Single Cell Analysis. This instrument will enable much more rapid and much cheaper sequencing than was previously possible at EI. The EI is an ideal environment for this instrument as it has access to automation for upstream sample prep. It has a highly trained workforce with a well-established training program and career progression for service delivery staff. EI also has very good IT infrastructure and a team of Bioinformaticians supporting the sequencing lab. Over a six month period the EI plan to install the platform, benchmark it and make it accessible to the UK bioscience community.

快速、廉价地对生物体基因组进行测序的能力已经彻底改变了生物学和生物医学研究。在过去的十年里，支持此类研究的技术得到了巨大的改进，我们理解数据的能力也得到了极大的提高。基因组测序已经从一项需要定制工厂规模设施的活动转变为需要一台可以在英国各地的大学实验室运行的仪器，现在甚至可以在手持设备中运行。然而，随着这个过程变得更快、更便宜，我们现在可以设计更大、更强大的研究，并且仍然需要定制设施来处理现在可能的更大规模的研究设计。在此，我们建议购买最新版本的短读长测序技术 NovaSeq 6000，并将其安装在 Earlham 研究所，该研究所负责 BBSRC 国家基因组学和单细胞分析能力的建设。该仪器将比 EI 以前实现的测序速度更快、成本更低。 EI 是该仪器的理想环境，因为它可以实现上游样品制备的自动化。它拥有一支训练有素的员工队伍，为服务提供人员提供完善的培训计划和职业发展。 EI 还拥有非常好的 IT 基础设施和支持测序实验室的生物信息学家团队。 EI 计划在六个月内安装该平台，对其进行基准测试，并使其可供英国生物科学界使用。

项目成果

Genomic and phylogenetic analysis of Salmonella Typhimurium and its monophasic variants responsible for invasive endemic infections in Colombia

导致哥伦比亚侵袭性地方性感染的鼠伤寒沙门氏菌及其单相变种的基因组和系统发育分析

• DOI: http://dx.10.1101/588608
• 发表时间: 2019
• 作者: Li Y

Extensive microbial diversity within the chicken gut microbiome revealed by metagenomics and culture.

宏基因组学和培养揭示了鸡肠道微生物组内广泛的微生物多样性。

• DOI: http://dx.10.7717/peerj.10941
• 发表时间: 2021
• 期刊: PeerJ
• 影响因子: 2.7
• 作者: Gilroy R