CIRCADIAN RETINAL MOTILITY ROLE OF CYCLIC NUCLEOTIDES

环核苷酸的昼夜视网膜运动作用

• 批准号: 3257941
• 负责人: MARY BETH BURNSIDE
• 金额: $ 15.59万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-12-01 至 1988-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3257941
• 关键词: autoradiography; biological information processing; calcium; catecholamines; circadian rhythms; cyclic AMP; cyclic GMP; dark adaptation; electrophoresis; eye movements; fresh water environment; histochemistry /cytochemistry; hormone regulation /control mechanism; light adaptations; phosphorylation; proteolysis; radioimmunoassay; retina; retinal pigment epithelium; tissue /cell culture; visual photoreceptor; visual pigments

The objective of this research program is to investigate the roles of cAMP, calcium, and hormones in the light- and circadian-regulation of teleost retinomotor movements. Retinomotor movements are produced by elongation and contraction of photoreceptors and migration of pigment granules within the RPE. They serve to position photoreceptors and screening pigment for optimal day or night vision. In constant darkness retinomotor movements persist; thus they appear to be regulated by circadian rhythm as well as light. We have shown that cAMP induces movements characteristic of darkness (night) in photoreceptors and RPE and that calcium is required for cone contraction. In this application we propose (1) to investigate the role of cAMP in promoting dark-adaptive retinomotor movements by examining dark- and cAMP-induced protein phosphorylation in RPE and in isolated photoreceptor fragments which contain inner and outer segments (IS/OS). The inner segments of these fragments are motile. (2) We will investigate the roles of calcium and calmodulin in regulating retinomotor movements by identifying calmodulin-binding proteins and examining calcium dependent protein phosphorylation in RPE and photoreceptor IS/OS. (3) We will investigate the effects of light on cAMP and cGMP levels in photoreceptor IS/OS by radioimmunoassay. (4) Finally we will examine the effects of hormones known to infuence cAMP levels on retinomotor movements in cultured retinas, on RPE and photoreceptor IS/OS cAMP levels, and on protein phosphorylation. We believe that information obtained from these studies will provide clues to the roles of these agents in regulating more general metabolic and physiological processes of photoreceptors and RPE in all species.

该研究计划的目的是调查CAMP的作用， 钙和昼夜节律调节中的激素 视网膜运动运动。 视网膜运动运动是通过伸长产生的 感光体的收缩和色素颗粒的迁移 RPE。 它们用于定位光感受器和筛选色素 最佳的白天或夜视。 在恒定的黑暗视网膜运动中 坚持;因此，它们似乎受昼夜节律和 光。 我们已经表明，营地引起了运动的特征 光感受器和RPE中的黑暗（夜），并且需要钙 锥收缩。 在此应用中，我们建议（1）调查 训练营在促进深色自适应视网膜运动运动中的作用 RPE和孤立的深色和cAMP诱导的蛋白质磷酸化 具有内部和外部段（IS/OS）的光感受器片段。 这些碎片的内部段是动的。 （2）我们将调查 钙和钙调蛋白在调节视网膜运动运动中的作用 鉴定钙调蛋白结合蛋白并检查钙依赖性 RPE和光感受器中的蛋白质磷酸化为/OS。 （3）我们会的 研究光对cAMP和CGMP水平感光受体的影响 IS/OS由放射免疫测定法。 （4）最后我们将研究 已知在培养的视网膜运动运动的激素已知 视网膜，在RPE和光感受器上是/OS营地，以及蛋白质 磷酸化。 我们相信从这些研究中获得的信息 将为这些代理在调节更一般的角色方面提供线索 所有感光者和RPE的代谢和生理过程 物种。