18-BTT: Epi-TOM: The development and characterization of an EpiRIL TOMato population

18-BTT：Epi-TOM：EpiRIL TOMato 群体的开发和表征

• 批准号: BB/S020837/1
• 负责人: Paul Fraser
• 金额: $ 25.76万
• 依托单位: Royal Holloway University of London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS020837%2F1
• 关键词: 18; BTT; Epi; TOM; development

Tomato (Solanum lycopersicum) is the most important commercial fruit crop in the world with an estimated global net production value well in excess of $50bn [www.fao.org]. On the basis of volume consumed (fresh and processed) tomato products play a crucial role in the human diet, providing essential minerals, vitamins and health promoting bioactive phytochemicals. Presently Marker Assisted Selection (MAS) has been the method of choice to deliver new varieties/hybrids with improved consumer and agronomic traits. MAS is reliant on the exploitation of natural variation. However, it has become apparent that in many crop species including tomato, phenotypic diversity is higher than that expected based on the available genetic variation alone. One explanation for this phenomena is the emerging evidence in plant species that stable inherited epigenetic variation can provide a basis for Darwinian evolution independent of DNA sequence changes.Using the model plant Arabidopsis, genetically identical recombinant inbred lines harbouring mosaics of methylation (epiRILs) have been created. This has been achieved by crossing two genetically identical lines with the exception that one of the parents is impaired in DNA methyltransferase MET1, a gene required for propagating cytosine methylation during DNA replication. In subsequent generations mosaics of methylation are fixed by self-pollination and single seed descent to F8. a unique epiRIL population in tomato of near homozygous lines with respect to their epigenomes, which will form the foundation of the current project and provide a valuable genetic/chemical community resource. The population consists of 115 lines. To date observable phenotypic differences are evident within the population (e.g. plant height and fruit size) However, the extent of chemical variation linked to consumer and agronomic traits awaits assessment within the remit of the proposed epi-TOM project. The project will deliver important information on the role of epigenetic variation on chemo/phenotypes in a complex genome, which can be exploited for the elucidation of novel quantitative traits with an epigenetic basis (epi-QTL).

番茄（Solanum lycopersicum）是世界上最重要的商业水果作物，估计全球净生产价值远超过500亿美元[www.fao.org]。根据消耗的数量（新鲜和加工）的番茄产品在人类饮食中起着至关重要的作用，提供必需的矿物质，维生素和促进生物活性植物化学物质的健康。目前的标记辅助选择（MAS）一直是具有改善消费者和农艺特征的新品种/杂种的首选方法。 MAS依赖于自然变异的剥削。但是，很明显，在包括番茄在内的许多农作物中，表型多样性高于仅基于可用的遗传变异的预期。对这种现象的一种解释是植物物种中的新兴证据表明，稳定的遗传遗传变异可以为达尔文进化提供独立于DNA序列变化的基础。使用模型植物拟南芥，遗传上相同的重组植物植物构成了携带甲基化镶嵌物（Epirils）的基因相同的重组植物。这是通过越过两个遗传相同的线路来实现的，但在DNA甲基转移酶met1中受损的一个父母是在DNA复制过程中传播胞嘧啶甲基化所需的基因。在随后的世代中，甲基化的镶嵌物是通过自花授粉和单一种子下降到F8的。相对于其表观基因组，番茄中的独特的Epiril人群将构成当前项目的基础，并提供有价值的遗传/化学群落资源。人口由115行组成。迄今为止，可观察到的表型差异在人群中显而易见（例如植物高度和水果大小），但是，与消费者和农艺性状相关的化学变异程度在拟议的Epi-Tom项目的职责中等待评估。该项目将提供有关表观遗传变异对化学/表型在复杂基因组中的作用的重要信息，该基因组可以用表观遗传基础（EPI-QTL）阐明新的定量性状。

项目成果

Isolation and characterization of sub-plastidial fractions from carotenoid rich fruits.

从富含类胡萝卜素的水果中分离和表征亚质体级分。

• DOI: 10.1016/bs.mie.2022.03.001
• 发表时间: 2022
• 期刊: Methods in enzymology
• 作者: Berry HM

Ketocarotenoid production in tomato triggers metabolic reprogramming and cellular adaptation: The quest for homeostasis?

番茄中酮类胡萝卜素的产生触发代谢重编程和细胞适应：寻求体内平衡？

• DOI: 10.1101/2023.01.09.523254
• 发表时间: 2023
• 作者: Nogueira M

Metabolomic approaches for the characterization of carotenoid metabolic engineering in planta.

用于表征植物中类胡萝卜素代谢工程的代谢组学方法。

• DOI: 10.1016/bs.mie.2022.02.017
• 发表时间: 2022
• 期刊: Methods in enzymology
• 作者: Perez-Fons L