Developing the Nanokick Bioreactor for Commercialisation and Cell Therapy

开发用于商业化和细胞治疗的 Nanokick 生物反应器

• 批准号: BB/S018808/1
• 负责人: Matthew Dalby
• 金额: $ 74.19万
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS018808%2F1
• 关键词: Developing; Nanokick; Bioreactor; Commercialisation; Cell

There is a >£1B market emerging for engineered bone graft, including products containing mesenchymal stem cells (MSCs). However, MSCs don't produce bone, osteoblasts do. Previous BBSRC funding has helped develop our Nanokick bioreactor for chemical-free supply of osteogenic, bone forming, cells. Here, we will address technical, regulatory and commercial objectives towards manufacture of a genuinely scalable allogeneic cell therapy for bone grafting. We have already secured funding for a first-in-man study in 2021. However, this project will address the regulatory and process specific technological challenges to supplying clinical grade cells for large trials and, ultimately, as a marketed therapeutic product. This same challenge of scalable manufacture has slowed or ceased progress of many cellular therapies, therefore the processes being developed here are expected to have wider impact in the cell therapy sector.

工程骨纹的出现了> 1B英镑的市场，包括含有间充质干细胞（MSC）的产品。但是，MSC不会产生骨骼，成骨细胞会。以前的BBSRC资金有助于开发我们的纳米基生物反应器，用于无化学化的成骨，骨形成，细胞。在这里，我们将解决技术，法规和商业目标，以制造真正可扩展的同种异体细胞疗法来进行骨骼移植。我们已经在2021年获得了一项人工研究的资金。但是，该项目将解决调查和处理特定技术挑战，以提供大型试验的临床级细胞，并最终作为销售的治疗产品。可扩展制造的同样挑战也减慢或停止了许多细胞疗法的进展，因此，此处开发的过程有望在细胞疗法领域产生广泛影响。

项目成果

Design, construction and characterisation of a novel nanovibrational bioreactor and cultureware for osteogenesis

用于成骨的新型纳米振动生物反应器和培养器皿的设计、构建和表征

• DOI: 10.1101/543660
• 发表时间: 2019
• 作者: Campsie P

Nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures.

• DOI: 10.1038/s41598-021-02139-9
• 发表时间: 2021-11-23
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Kennedy JW;Tsimbouri PM;Campsie P;Sood S;Childs PG;Reid S;Young PS;Meek DRM;Goodyear CS;Dalby MJ
• 通讯作者: Dalby MJ

Reduction of Pseudomonas aeruginosa biofilm formation through the application of nanoscale vibration.

通过应用纳米级振动减少铜绿假单胞菌生物膜的形成。

• DOI: 10.1016/j.jbiosc.2019.09.003
• 发表时间: 2020
• 期刊: Journal of bioscience and bioengineering
• 影响因子: 2.8
• 作者: Robertson SN

The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells.

• DOI: 10.1126/sciadv.abb7921
• 发表时间: 2021-03
• 期刊: Science advances
• 影响因子: 13.6
• 作者: Hodgkinson T;Tsimbouri PM;Llopis-Hernandez V;Campsie P;Scurr D;Childs PG;Phillips D;Donnelly S;Wells JA;O'Brien FJ;Salmeron-Sanchez M;Burgess K;Alexander M;Vassalli M;Oreffo ROC;Reid S;France DJ;Dalby MJ
• 通讯作者: Dalby MJ

Hurdles to uptake of mesenchymal stem cells and their progenitors in therapeutic products.

• DOI: 10.1042/bcj20190382
• 发表时间: 2020-09-18
• 期刊: The Biochemical journal
• 作者: Childs PG;Reid S;Salmeron-Sanchez M;Dalby MJ
• 通讯作者: Dalby MJ